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Luciferase Reporter Assays The circ-000543 wild sequence, ZNF268-3′UTR wild sequence and their mutations with or without miR-567 binding sites were synthesized and then subcloned into luciferase reporter vectors psiCHECK-2, which was bought from Promega (Madison, WI, USA). HEK293T cells were implanted in 24-well plates at the density of 3×10 4 cells/well in triplicate. Then, miR-567 or NC mimic were cotransfected with corresponding psiCHECK-2 plasmids. After co-transfection for 48 h, dual-luciferase reporter assay system that provided by Promega was proceeded to conduct the luciferase repo...

Purpose: Asthma and chronic obstructive pulmonary disease (COPD) are complex and heterogeneous inflammatory diseases. We sought to investigate distinct disease profiles based on clinical, cellular and molecular data from patients with mild-to-moderate obstructive pulmonary diseases. Patients and Methods: Patients with mild-to-moderate allergic asthma (n=30) and COPD (n=30) were prospectively recruited. Clinical characteristics and induced sputum were collected. In total, 35 mediators were assessed in induced sputum. Logistic regression analysis was conducted to identify the optimal factors th...

CircRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: selected key circRNAs in neuroblastoma and their associations with clinical features

Elsevier Announces Free Issue to Support IWD 2021 Starting today, over 50 carefully selected journal articles and book chapters will be publicly accessible to raise awareness on issues of women and gender in health and science NEW YORK, NY / ACCESSWIRE / March 8, 2021 Starting today, over 50 carefully selected journal articles and book chapters will be publicly accessible to raise awareness on issues of women and gender in health and science Elsevier, a global leader in research publishing and information analytics, has launched a free access special issue of curated content focused on women ...

AAP PROSE Awards: The 2021 Category Winners From biological science and ‘The Ethical Algorithm’ to legal studies and ‘Demagogue for President,’ the AAP PROSE category winners embrace 45 fields of study. The apse mosaic of Sant’ Apollinare in Classe, Ravenna’s patron saint. Judith Herrin’s ‘Ravenna: Capital of Empire Crucible of Europe’ from Princeton University Press has won the 2021 European History category PROSE Award. Image – iStockphoto: Sergio Delle Vedove Swann: ‘Exceptional Scholarship’ You’ll remember Today (January 28), we have the winners in those 45 subj...