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Lab-on-a Chip Expands Functional Studies of Enzyme Variants


Lab-on-a Chip Expands Functional Studies of Enzyme Variants
July 22, 2021
A microfluidic platform for high-throughput expression, purification, and characterization of enzyme variants has been applied to the study of a familiar enzyme, PafA (phosphate-irrepressible alkaline phosphatase of Flavobacterium). [Daniel Mokhtari, Stamford University]
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Just because an enzyme has an active site doesn’t mean the rest of the enzyme should be disregarded, as though it were so much dead weight. It influences enzyme function, if only by keeping the enzyme from unraveling and losing its function altogether. The rest of the enzyme may also fine-tune enzyme function in ways that are rather more subtle too subtle, alas, to be determined without the patient study of enzyme variants, that is, versions of the enzyme that incorporate one or more mutations. Because studying how mutations affect enzyme function is such a painstaking business, the mutations that are assessed tend to ....

Polly Fordyce , Dan Herschlag , Stanford School Of Medicine , Stanford University , High Throughput Microfluidic Enzyme Kinetics , Streetlight Effect , ஸ்டான்போர்ட் பள்ளி ஆஃப் மருந்து , ஸ்டான்போர்ட் பல்கலைக்கழகம் , தெரு விளக்கு விளைவு ,

The inner workings of an enzyme

The inner workings of an enzyme
sciencemag.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from sciencemag.org Daily Mail and Mail on Sunday newspapers.

High Throughput Microfluidic Enzyme Kinetics ,

New tool drastically speeds up the study of enzymes

New tool drastically speeds up the study of enzymes
stanford.edu - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from stanford.edu Daily Mail and Mail on Sunday newspapers.

Polly Fordyce , Craig Markin , Daniel Mokhtari , Dan Herschlag , Stanford School Of Medicine , Stanford University , High Throughput Microfluidic Enzyme Kinetics , ஸ்டான்போர்ட் பள்ளி ஆஃப் மருந்து , ஸ்டான்போர்ட் பல்கலைக்கழகம் ,

Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics
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High Throughput Microfluidic Enzyme Kinetics ,