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In this application note, Oxford Nanopore Technologies describes how it has developed a sequencing protocol for the analysis of longer DNA fragments. This approach provides comprehensive genomic insights, including the detection of structural variants, copy number variations, and methylation patterns. Obtaining complete genome information is essential for understanding chromosome function, human diseases, and genomic variation. The integration of the Ligation Sequencing Kit XL V14 on the DreamPr ....
The Tecan Group and Oxford Nanopore Technologies plc (Oxford Nanopore) today announce their collaboration to drive easier high-throughput nanopore library preparation in an automated, hands-free fashion. ....
Abstract Alternative mRNA isoforms play a key role in generating diverse protein isoforms. To dissect isoform usage in the subcellular compartments of single cells, we introduced an novel approach, nanopore sequencing coupled with single-cell integrated nuclear and cytoplasmic RNA sequencing, that couples microfluidic fractionation, which separates cytoplasmic RNA from nuclear RNA, with full-length complementary DNA (cDNA) sequencing using a nanopore sequencer. Leveraging full-length cDNA reads, we found that the nuclear transcripts are notably more diverse than cytoplasmic transcripts. Our findings also indicated that transcriptional noise emanating from the nucleus is regulated across the nuclear membrane and then either attenuated or amplified in the cytoplasm depending on the function involved. Overall, our results provide the landscape that shows how the transcriptional noise arising from the nucleus propagates to the cytoplasm. ....