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Antibiotic resistant bacteria-associated infections are responsible for more than 1.2 million annual deaths worldwide. In low- and middle-income countries (LMICs), the consumption of antibiotics for human and veterinary uses is not regulated effectively. Overused and misused antibiotics can end up in aquatic environments, which may act as a conduit for antibiotic resistance dissemination. However, data on the prevalence of antibiotic resistance determinants in aquatic environments are still limited for LMICs. In this study, we evaluated the prevalence and concentration of antibiotic resistance genes in different drinking and environmental water sources collected from the Kathmandu Valley, Nepal using droplet digital PCR to understand the current situation of ARG contamination. River water and shallow dug well water were the most contaminated with ARGs. Almost all samples contained sul1 (95%), and intI1 and tet(A) were detected in 83% and 60% of the samples, respectively. Maximum ARG co ....

New York , United States , France General , Kathmandu Valley , Nepal General , Hanumante River , Bishnumati River , Godavari River , Al Munastir , Japan General , Sarmila Tandukar , Kazi Tamrakar , Args Gillings , Rajani Ghaju Shrestha , Lmics Devarajan , Robles Aguilar , Van Boeckel , Pcr Evagreen Supermix , Ghaju Shrestha , Dinesh Bhandari , National Population , Canadian Paediatric Society , Rr Core Team , Cultural Organization , Technology Agency , Japan Society For The Promotion Of Science ,