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Enterovirus detection in different regions of Madagascar reveals a higher abundance of enteroviruses of species C in areas where several outbreaks of vaccine-derived polioviruses occurred | BMC Infectious Diseases

Poliomyelitis outbreaks due to pathogenic vaccine-derived polioviruses (VDPVs) are threatening and complicating the global polio eradication initiative. Most of these VDPVs are genetic recombinants with non-polio enteroviruses (NPEVs) of species C. Little is known about factors favoring this genetic macroevolution process. Since 2001, Madagascar has experienced several outbreaks of poliomyelitis due to VDPVs, and most of VDPVs were isolated in the south of the island. The current study explored some of the viral factors that can promote and explain the emergence of recombinant VDPVs in Madagascar. Between May to August 2011, we collected stools from healthy children living in two southern and two northern regions of Madagascar. Virus isolation was done in RD, HEp-2c, and L20B cell lines, and enteroviruses were detected using a wide-spectrum 5ʹ-untranslated region RT-PCR assay. NPEVs were then sequenced for the VP1 gene used for viral genotyping. Overall, we collected 1309 stools, ....

United Kingdom , Languedoc Roussillon , Beckman Coulter Genomics Company Genewiz , Global Polio Laboratory Network , Modified Eagle Medium , Polio Laboratory Network , Intratypic Detection , Roche Diagnostics , Beckman Coulter Genomics Company , Genewiz Company , Codoncode Aligner , Maximum Likelihood ,

Identification and Genetic Characterization of Fasciola hepatica Isola

Identification and Genetic Characterization of Fasciola hepatica Isola
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Saudi Arabia , Red Sea , Djibouti General , United States , United Kingdom , South Africa , Ar Riya , City Of , Al Taif , Saudi Arabian , World Health Organization , South America , Jeddah Province , Jeddah Municipal , Life Technologies , Chain Reaction , Mastercycler Personal , Bioline Isolate , Maximum Likelihood , Sequences Used , Worms Isolated , Distances Among , Present Study Based , Sequence Analysis , Eastern Europe , North Africa ,

Vulnerability as an Ex Ante Measure of Poverty : An Empirical Investigation for India

Using both rounds of the India Human Development Survey, vulnerability is measured as an ex ante measure of poverty for the Indian households. This article highlights the importance of measuring vulnerability in the overall poverty calculation and found it to be a significant predictor of the future poverty. ....

New York , United States , United Kingdom , New Delhi , Republic Of , Columbia University , City Of , Katsushi Imai , Richard Boisvert , Agnes Quisumbing , Woonjin Kang , Martin Ravallion , Asep Suryahadi , Jyotsna Jalan , Suresh Tendulkar , Raghav Gaiha , Reeve Vanneman , Geoff Wood , Kenneth Harttgen , Onkar Mittal , Department For International Development Loughhead , National Sample Survey Office , University Of Maryland , Oxford Development , European Journal Of Development Research , National Council Of Applied Economic Research ,

ERG11 Gene of Fluconazole-resistant Candida albicans

Our results showed new mutations in the heme-binding pocket of caCYP51 that explain the resistance to fluconazole exhibited by the Candida isolates. ....

United States , France General , Nordrhein Westfalen , Andhra Pradesh , Nepal General , Candida Agar Chromagar , Clustal Omega , Laboratory Standards Institute , National Center , Protein Data Bank , University Of Nigeria Medical Center , Molecular Evolutionary Genetic Analysis Software , University Of Nigeria Veterinary Teaching Hospital , Plain Language , Nigeria Medical Center , Nigeria Veterinary Teaching Hospital , Sabouraud Dextrose Agar , Chromogenic Agar , Candida Agar , Genomic Lysis Buffer , Lysis Buffer , Wash Buffer , Candida Species , Thermal Cycler , Thermo Fisher Scientific , Zymo Seq ,

Lineages of monkeypox virus in the United States

In a recent work posted to the bioRxiv preprint server, researchers analyzed various monkeypox virus (MPXV) lineages identified recently in the United States (US). ....

United States , Image Credit , Congo Basin , West African , Supplemental Table , Median Joining , Monkeypox West African Selective , Maximum Likelihood , Genomic Sequencing , Single Nucleotide Polymorphism ,