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A fast, highly sensitive, point-of-care RT-LAMP and CRISPR/Cas12a assay for the detection of SARS-CoV-2

Researchers used isothermal amplification with CRISPR-Cas12a to detect SARS-CoV-2. ....

Research Square , Coronavirus Disease Covid 19 , Ars Cov 2 , Covid 19 , Palindromic Repeats , Polymerase Chain Reaction , Evere Acute Respiratory , Evere Acute Respiratory Syndrome ,

CRISPR-based diagnostics for SARS-CoV-2 detection

In a recent study posted to the Journal of Biochemical and Molecular Toxicology, researchers reviewed clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). ....

Meletios Verras , Institute Of Genomics , Molecular Toxicology , Integrative Biology , Coronavirus Disease Covid 19 , Ars Cov 2 , Covid 19 , Gold Nanoparticles , Palindromic Repeats , Polymerase Chain Reaction , Public Health , Ibonucleic Acid , Evere Acute Respiratory , Evere Acute Respiratory Syndrome ,

New method increases efficiency of gene editing while minimizing DNA deletion sizes

Wake Forest Institute for Regenerative Medicine (WFIRM) scientists working on CRISPR/Cas9-mediated gene editing technology have developed a method to increase efficiency of editing while minimizing DNA deletion sizes, a key step toward developing gene editing therapies to treat genetic diseases. ....

Anthony Atala , Baisong Lu , Emily Henderson , Us Department Of Defense , Wake Forest Institute For Regenerative Medicine , Nucleic Acids Research , Wake Forest University Health Sciences , Forest Institute , Regenerative Medicine , North Carolina , Forest University Health Sciences , Rna Polymerase , Genome Editing , Palindromic Repeats ,

CRISPR/Cas9 deletions induce adverse on-target genomic effects

Researchers utilized a CRISPR/Cas9 system to evaluate the usage of tRNA by deleting two tRNA genes from the genomes of hyper hepatocellular carcinoma and human near-haploid chronic myeloid leukemia cells. ....

Oxford Nanopore Technology , Amino Acid , Palindromic Repeats ,

CRISPR-based detection of SARS-CoV-2 RNA directly from patient swabs

Researchers developed a clustered regularly interspaced short palindromic repeats-based diagnostic technique for detecting SARS-CoV-2 RNA. ....

Thermusthermophilus Ttcsm , Research Square , Coronavirus Disease Covid 19 , Ars Cov 2 , Covid 19 , Palindromic Repeats , Polymerase Chain Reaction , Ibonucleic Acid , Rna Extraction , Evere Acute Respiratory , Evere Acute Respiratory Syndrome ,