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9 Aberrantly expressed CCDC144NL-AS1 was observed in gastric cancer,
9 however, its roles in HCC development and the potential mechanism have not been reported yet. In this study, we investigated the expression pattern of CCDC144NL-AS1 using human HCC tissues, and explored the underlying mechanism from the epigenetic pathway. We also estimated the therapy value of CCDC144NL-AS1 using HCC mouse model. Our study may provide novel therapeutic targets for HCC treatment to improve the prognosis of HCC.
Patients and Methods
Tissue Collection
From April 2014 to December 2015, 135 pairs of HCC tissues and the matched adjacent normal tissues were collected from the Department of Infectious Diseases of the First Affiliated Hospital of Zhengzhou University. Patients who received treatments before or complicated with distant metastasis or other systemic diseases such as diabetes, cardiovascular, or cerebrovascular diseases were excluded from this study. ....

Eteläuomen Läi , United States , Streptavidin Invitrogen , Becton Dickinson , Public Protein Plasmid Library Jiangsu , Tecan Group Ltd , Proteintech Group , Imagej National Institutes Of Health , Cell Signaling Technology , First Affiliated Hospital Of Zhengzhou University , Graphpad Software , Keygen Biotech Co Ltd , Infectious Diseases , First Affiliated Hospital , Ethics Committee , Zhengzhou University , American Type Culture Collection , Modified Eagle , Thermo Fisher Scientific , Transfection Reagent , Plasmid Library , Animal Experiments , Multi Site Directed Mutagenesis Kit , National Institutes , Bio Rad Laboratories , Deep Sequencing ,