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Expanded geographic distribution and host preference of Anopheles gibbinsi (Anopheles species 6) in northern Zambia | Malaria Journal

Nchelenge District in northern Zambia suffers from holoendemic malaria transmission despite a decade of yearly indoor residual spraying (IRS) and insecticide-treated net (ITN) distributions. One hypothesis for this lack of impact is that some vectors in the area may forage in the early evening or outdoors. Anopheles gibbinsi specimens were identified in early evening mosquito collections performed in this study area, and further insight was gleaned into this taxon, including characterizing its genetic identity, feeding preferences, and potential role as a malaria vector. Mosquitoes were collected in July and August 2019 by CDC light traps in Nchelenge District in indoor sitting rooms, outdoor gathering spaces, and animal pens from 16:00–22:00. Host detection by PCR, COI and ITS2 PCR, and circumsporozoite (CSP) ELISA were performed on all samples morphologically identified as An. gibbinsi, and a subset of specimens were selected for COI and ITS2 sequencing. To determine risk facto ....

United States , New Zealand , Nchelenge District , Kent Norris , National Center , Reference Reagent Resource Center , Germany At Purdue University , Light Traps Johnw Hock Co , Biomatters Ltd , Johns Hopkins Bloomberg School Of Public Health , University Of The Witwatersrand , Resources Malaria Research , Lake Mweru , Johns Hopkins Bloomberg School , Public Health , Qiagen Tissuelyser , Purdue University , Johns Hopkins Medical Institutions , Geneious Prime , Biotechnology Information , New England Biolabs , Malaria Research ,

Interdependence of a mechanosensitive anion channel and glutamate receptors in distal wound signaling

Interdependence of a mechanosensitive anion channel and glutamate receptors in distal wound signaling
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