This paper proposed and prototyped a metamaterial isolator with periodic structure based on magnetorheological elastomer (MRE) and magnet spring. With acoustic metamaterial structure, the proposed metamaterial isolator can generate a stop-band, which means the vibration over specific frequency can't be transferred and will be isolated effectively. Then, with the control of MRE stiffness, the cut-off frequency of the stop-band is tunable, which makes the isolator potential to be adapted to different working situations. The negative stiffness generated by the magnet spring can help to lower...