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Researchers use single-cell RNA sequencing technologies to track cellular development in leaves

Researchers use single-cell RNA sequencing technologies to track cellular development in leaves
How do we become a complex, integrated multicellular organism from a single cell?
While developmental biologists have long researched this fundamental question, Stanford University biologist and HHMI investigator Dominique Bergmann s recent work on the plant Arabidopsis thaliana has uncovered surprising answers.
In a new study, published April 5 in
Developmental Cell, led by Bergmann and postdoctoral scholar Camila Lopez-Anido, researchers used single-cell RNA sequencing technologies to track genetic activity in nearly 20,000 cells as they formed the surface and inner parts of an Arabidopsis leaf.
Through this highly detailed technique, the researchers captured transient and rare cell states and found a surprising abundance of ambiguity in how cells traversed various identities, particularly early on within the stem cell population. ....

United States , Yan Gong , Camila Lopez Anido , Maria Ximena Anleu Gil , Emily Henderson , Annika Weimer , Dominique Bergmann , Leonardw Ely Jr , Nidhi Sharma , N Katherine Smoot , Stanford Bio , Georges Seurat , National Institutes Of Health , School Of Humanities , Bard College , German Research Foundation , Stanford Cancer Institute , Stanford University , Reed College , Howard Hughes Medical Institute , Developmental Cell , Study Senior Author , Virginia Lopez Anido , Citizen Science , Anne Vat , Victoria Guo ,

An artful study of cellular development in leaves

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IMAGE: A drawing series by artist Virginia Lopez-Anido that was inspired by research by her sister, Camila Lopez-Anido, a postdoctoral scholar at Stanford who studies cellular development in plants.
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Credit: Virginia Lopez-Anido
How do we become a complex, integrated multicellular organism from a single cell?
While developmental biologists have long researched this fundamental question, Stanford University biologist and HHMI investigator Dominique Bergmann s recent work on the plant Arabidopsis thaliana has uncovered surprising answers.
In a new study, published April 5 in
Developmental Cell, led by Bergmann and postdoctoral scholar Camila Lopez-Anido, researchers used single-cell RNA sequencing technologies to track genetic activity in nearly 20,000 cells as they formed the surface and inner parts of an Arabidopsis leaf. Through this highly detailed technique, the researchers captured transient and rare cell states and foun ....

United States , Yan Gong , Camila Lopez Anido , Maria Ximena Anleu Gil , Annika Weimer , Dominique Bergmann , Leonardw Ely Jr , Nidhi Sharma , Stanford Bio , N Katherine Smoot , Georges Seurat , National Institutes Of Health , School Of Humanities , Bard College , German Research Foundation , Stanford Cancer Institute , Stanford University , Reed College , Howard Hughes Medical Institute , Developmental Cell , Virginia Lopez Anido , Citizen Science , Anne Vat , Victoria Guo , Stanford Bio X , Stanford Cancer ,