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Breast cancer–secreted factors perturb murine bone growth in regions prone to metastasis


Abstract
Breast cancer frequently metastasizes to bone, causing osteolytic lesions. However, how factors secreted by primary tumors affect the bone microenvironment before the osteolytic phase of metastatic tumor growth remains unclear. Understanding these changes is critical as they may regulate metastatic dissemination and progression. To mimic premetastatic bone adaptation, immunocompromised mice were injected with MDA-MB-231–conditioned medium [tumor-conditioned media (TCM)]. Subsequently, the bones of these mice were subjected to multiscale, correlative analysis including RNA sequencing, histology, micro–computed tomography, x-ray scattering analysis, and Raman imaging. In contrast to overt metastasis causing osteolysis, TCM treatment induced new bone formation that was characterized by increased mineral apposition rate relative to control bones, altered bone quality with less matrix and more carbonate substitution, and the deposition of disoriented mineral near the g ....

United States , Baden Wuberg , Witec Gmb , Ctan Bruker , Broad Institute , Rx Solutions , Developmental Studies Hybridoma Bank , Cornell University Institutional Animal Care , Graphpad Software , Cornell University , Institutional Animal Care , American Type Culture Collection , Life Technologies , Preparation Kit , Illumina Nextseq , Thermo Fisher Scientific , Vector Laboratories , Mouse On Kit , Toptica Photonics , Graphpad Prism , Creative Commons Attribution Noncommercial , ஒன்றுபட்டது மாநிலங்களில் , பரந்த நிறுவனம் , ர்க்ஸ் தீர்வுகள் , கார்னெல் பல்கலைக்கழகம் நிறுவன விலங்கு பராமரிப்பு , கார்னெல் பல்கலைக்கழகம் ,