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A Guide to Particle Size Distribution Weighting


Some particle size instruments, known as single particle counters, measure individual particle size, while others measure surface area as a function of particle size. Further instruments measure mass or volume versus size, while others still measure multiple functions of scattered light intensity as a function of size.
Each of these instruments can generate particle size distributions and, in theory, one can transform between types in order to correlate the results. In practice, if a measurement with a single particle counter generated a differential number-weighted size distribution, how would one undertake a comparison of the results with a measurement deriving from a different type of instrument, which generated a differential volume-weighted size distribution? ....

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Using Dynamic Light Scattering (DLS) to Analyze Virus Particles


Using Dynamic Light Scattering (DLS) to Analyze Virus Particles
Conventional visual techniques of detecting virus particles in solution provide a glimpse of an extremely small sampling volume, while the method of particle analysis employing dynamic light scattering generates an ensemble
average of particles inside the solutions.
Virus particles incubated in the laboratory pose an issue, as they need to be grown inside cells in a media containing albumin and additional small proteins, like those inside Fetal Bovine Serum (FBS) or Minimum Essential Media (MEM) solutions.
When virus particles are emitted from the cells, the cell pieces are big and can be divided by centrifugation, however the lesser proteins of the media cannot be eliminated. Cautious choice of the distribution parameters from a dynamic light scattering experiment permits clear observation of the size distributions of virus particles in the presence of significantly smaller proteins that make up the ....

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Using Dynamic Light Scattering (DLS) to Analyze Virus Particles


Using Dynamic Light Scattering (DLS) to Analyze Virus Particles
Conventional visual techniques of detecting virus particles in solution provide a glimpse of an extremely small sampling volume, while the method of particle analysis employing dynamic light scattering generates an ensemble
average of particles inside the solutions.
Virus particles incubated in the laboratory pose an issue, as they need to be grown inside cells in a media containing albumin and additional small proteins, like those inside Fetal Bovine Serum (FBS) or Minimum Essential Media (MEM) solutions.
When virus particles are emitted from the cells, the cell pieces are big and can be divided by centrifugation, however the lesser proteins of the media cannot be eliminated. Cautious choice of the distribution parameters from a dynamic light scattering experiment permits clear observation of the size distributions of virus particles in the presence of significantly smaller proteins t ....

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