E-Mail IMAGE: a, Schematic of the setup. Full-frame spectral micrographs are obtained by the synchronized fast modulation of the excitation wavelength in consecutive frames. P, polarizer; L, lens; F, bandpass filter; DM,... view more Credit: by Kun Chen, Rui Yan, Limin Xiang, and Ke Xu The multiplexing capability of fluorescence microscopy is severely limited by the broad fluorescence spectral width. Spectral imaging offers potential solutions, yet typical approaches to disperse the local emission spectra notably impede the attainable throughput and place substantial constraints on temporal resolution. Tunable bandpass filters provide a possibility to scan through the emission wavelength in the wide field. However, applying narrow bandpasses to the fluorescence emission results in inefficient use of the scarce signal.