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>> there are a few to lead this research, naturally occurring mutations which naturally give gain function and investigative study these effects of interest. does this mutation make something more transmissible, more pathogenic or adapt. or, historically investigators have done to actually create gain of function by making mutations adaptations of the newark genetic techniques and genetic reinforcement. historically investigated was to actually create function by making mutations pathogenic and add new genetic techniques such as reverse genetics . >> reverse genetics or technique used by scientists to create

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