Understanding Absolute Stoichiometry of Oligomeric Protein Complexes Using SEC-MALS Thought LeadersSheena D'Arcy & Prithwijit SarkarD'Arcy Group University of Texas In this interview, Sheena D'Arcy and Prithwijit Sarkar from the D'Arcy Group in the Department of Chemistry and Biochemistry at the University of Texas at Dallas talks to News-Medical Life Sciences about using SEC-MALS to characterize the native oligomeric state of Nap proteins and their complexes. What is SEC-MALS? SEC-MALS is size-exclusion chromatography coupled to multi-angle light scattering. The SEC component is comprised of an HPLC or FPLC pump and SEC column plus a UV absorption detector. An aliquot of solution that typically contains a mixture of different protein species is injected and flows through the packed column.