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Coinfection with PEDV and BVDV induces inflammatory bowel disease pathway highly enriched in PK-15 cells | Virology Journal

From the 1078 diarrhea stools tested in our survey from 2017 to 2020 in local area of China, PEDV was the key pathogen that was closely related to the death of piglets with diarrhea. In addition, coinfection of PEDV-positive samples with BVDV reached 17.24%. Although BVDV infection in swine is typically subclinical, the effect of PEDV and BVDV coinfection on disease severity and the potential molecular mechanism of coinfection with these two viruses remain unknown. In this study, we developed a model of coinfection with porcine epidemic diarrhea virus (PEDV) and bovine viral diarrhea virus (BVDV) in PK15 cells, and a tandem mass tag (TMT) combined with LC–MS/MS proteomic approach was used to identify differential protein expression profiles. Additionally, we performed drug experiments to explore the inflammatory response induced by PEDV or BVDV mono- or coinfection. A total of 1094, 1538, and 1482 differentially expressed proteins (DEPs) were identified upon PEDV monoinfection, B ....

Yang Zhou , United States , United Kingdom , Baden Wüberg , South Korea , City Of , Bionote Hwaseong Si , Guoqiang Zhu , Cell Signaling Technology Danvers , Novogene Bioinformatics Technology Co Ltd , Yang Zhou University , Carl Zeiss , Grand Island , Cell Signaling Technology , Bioss Biotechnology , Western Blot , Alexa Fluor , Thermo Fisher Scientific , Beyotime Biotechnology , Trypsin Gold , Proteome Discoverer , Matrix Science , Kyoto Encyclopedia , Dual Luciferase Reporter Assay System ,

Safety and immunogenicity of two recombinant DNA COVID-19 vaccines containing the coding regions of the spike or spike and nucleocapsid proteins: an interim analysis of two open-label, non-randomised, phase 1 trials in healthy adults

Safety and immunogenicity of two recombinant DNA COVID-19 vaccines containing the coding regions of the spike or spike and nucleocapsid proteins: an interim analysis of two open-label, non-randomised, phase 1 trials in healthy adults
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Soult Ukpyolsi , South Korea , Eteläuomen Läi , Ch Ungch Ong Bukto , Kyonggi Do , Bionote Hwaseong Si , Korea Ministry Of Food , Korean Ministry Of Food , National Culture Collection For Pathogen , Severance Hospital , Gangnam Severance , Good Clinical , Korea Ministry , Drug Safety , Institutional Review Board , South Korean Ministry , Common Terminology Criteria , Adverse Events , Medical Dictionary , Regulatory Activities , National Culture Collection ,