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September HPC User Forum Event to Include Latest in AI in HPC, Sustainability, and Cloud Computing

September HPC User Forum Event to Include Latest in AI in HPC, Sustainability, and Cloud Computing
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Towards the rate limit of heterologous biotechnological reactions in recombinant cyanobacteria | Biotechnology for Biofuels and Bioproducts

Cyanobacteria have emerged as highly efficient organisms for the production of chemicals and biofuels. Yet, the productivity of the cell has been low for commercial application. Cyanobacterial photobiotransformations utilize photosynthetic electrons to form reducing equivalents, such as NADPH-to-fuel biocatalytic reactions. These photobiotransformations are a measure to which extent photosynthetic electrons can be deviated toward heterologous biotechnological processes, such as the production of biofuels. By expressing oxidoreductases, such as YqjM from Bacillus subtilis in Synechocystis sp. PCC 6803, a high specific activity was obtained in the reduction of maleimides. Here, we investigated the possibility to accelerate the NAD(P)H-consuming redox reactions by addition of carbohydrates as exogenous carbon sources such as D-Glucose under light and darkness. A 1.7-fold increase of activity (150 µmol min−1 gDCW−1) was observed upon addition of D-Glucose ....

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