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Technologies enable 3D imaging of whole human

Technologies enable 3D imaging of whole human
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Soult Ukpyolsi , South Korea , United States , Chang Sin , Kangwon Do , Qiangge Zhang , Elast Webster Guan , Larsa Gjesteby , Slayton Marx , C Dirk Keene , Nicholasb Evans , Satrajits Ghosh , Matthew Frosch , Ji Wang , Kwanghun Chung , Chuanxi Zhao , Lauraj Brattain , Xinyi Gu , Guoping Feng , Mira Moukheiber , Minyounge Kim , X William Yang , Lee Kamentsky , David Chavez , Jeff Stirman , Patrickr Hof ,

"SCOUT" helps researchers find, quantify significant differences among organoids | MIT News


Caption:
This cross-section of an organoid processed with SCOUT shows cells labeled with various antibodies to highlight different cell types: cell nuclei are in blue (DAPI dye), glia/neural progenitor nuclei are in red (anti-SOX2), glia/neural progenitor projections are in green (anti-vimentin), neuronal projections are in white (anti-B3-tubulin).
Credits:
Image courtesy of the Chung lab.
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The ability to culture cerebral organoids, or “minibrains,” using stem cells derived from people has given scientists experimentally manipulable models of human neurological development and disease, but not without confounding challenges. No two organoids are alike and none of them resemble actual brains. This “snowflake” problem has held back the science by making scientifically meaningful quantitative comparisons difficult to achieve. To help researchers overcome those ....

Dae Hee Yun , Nicholasb Evans , Silvia Velasco , Kwanghun Chung , Justin Swaney , Paola Arlotta , Alexandre Albanese , Jennam Antonucci , Lee Gehrke , Cultural Foundation , Harvard University , Institute For Medical Engineering , National Institutes Of Health , Cognitive Sciences , Picower Institute For , Unbiased Techniques , Associate Professor Kwanghun , Picower Institute , Medical Engineering , Chemical Engineering , Helmholtz Professor , National Institutes , டே ஹீ யுன் , சில்வியா வெலாஸ்கோ , ஜஸ்டின் ஸ்வானே , அலெக்ஸாண்ட்ரே அல்பானீஸ் ,

"SCOUT" helps researchers find, quantify significant differences among organoids


Massachusetts Institute of Technology
Unbiased, high-throughput analysis pipeline improves utility of “minibrains” for understanding development and diseases such as Zika infection.
This cross-section of an organoid processed with SCOUT shows cells labeled with various antibodies to highlight different cell types: cell nuclei are in blue (DAPI dye), glia/neural progenitor nuclei are in red (anti-SOX2), glia/neural progenitor projections are in green (anti-vimentin), neuronal projections are in white (anti-B3-tubulin).
Image courtesy of the Chung lab.
The ability to culture cerebral organoids, or “minibrains,” using stem cells derived from people has given scientists experimentally manipulable models of human neurological development and disease, but not without confounding challenges. No two organoids are alike and none of them resemble actual brains. This “snowflake” problem has held back the science by making sc ....

Dae Hee Yun , Nicholasb Evans , Silvia Velasco , Kwanghun Chung , Justin Swaney , Paola Arlotta , Alexandre Albanese , Jennam Antonucci , Lee Gehrke , Cultural Foundation , Harvard University , Institute For Medical Engineering , National Institutes Of Health , Cognitive Sciences , Picower Institute For , Unbiased Techniques , Associate Professor Kwanghun , Picower Institute , Medical Engineering , Chemical Engineering , Helmholtz Professor , National Institutes , டே ஹீ யுன் , சில்வியா வெலாஸ்கோ , ஜஸ்டின் ஸ்வானே , அலெக்ஸாண்ட்ரே அல்பானீஸ் ,