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Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L. plantarum was optimized for PMA treatment concentration, PMA detection sensitivity and multiple con...

Targeting Unsaturated Fatty Acids to Reduce Scalp Inflammation—A Proposal February 1, 2021 Contact Author Xianting Lin and Misa Nakamura, Beauty Hi-tech Innovation Co., Ltd.; Yalin Wang, Yunnan Baiyao Group Healthcare Products Co., Ltd.; and Jianzhong Yang, Port Island, Kobe, Japan Close Sponsored The human scalp can be likened to soil in which the hair grows like plants. If the scalp is kept healthy, the hair will grow and flourish—and sebum is one important aspect of this environment, as it keeps the scalp moisturized. However, there is a strong connection between scalp infl...