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T cell expansion and viability using Stericup-filtered media

In this application note, Merck emphasizes the importance of contamination-free T cell culture media prepared through filter sterilization. The right filter membrane retains essential growth factors while removing contaminants. The Stericup ® filtration systems maintain protein integrity and minimize waste. The study examines repeated filtration of T cell media using Stericup filters, showing sustained T cell growth and function, aiding T cell therapy production. ....

Application Note , Cell Culture , T Cell ,

Validation of Stericup filtration systems for stem cell culture

In this application note, Merck demonstrates that microporous filtration, despite being the chosen method to prepare and minimize contamination in cell culture processes, raises apprehension about potential loss of valuable factors and addition of harmful components to growth media. This concern is particularly relevant in stem cell culture due to the cost of factors, sensitivity of cells to additives, and extended culture durations. The study reveals that using a polyethersulfone (PES) membrane ....

Application Note , Cell Culture , Stem Cell ,