[Full text] Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Pe
Figure 1 Construction of occludin/ZO1 probes and analysis of their efficacy and motility. ( A) In cpstFRET (cpVenus-7aa-cpCerulean), cpCerulean (cyan) is the donor and cpVenus (yellow) is the acceptor. The probes have high Förster resonance energy transfer (FRET) efficiency when donor and acceptor are parallel without external force (f: external force). The external force reduces the FRET efficiency by changing the angle of cpstFRET. ( B) Schematic representation of the occludin/ZO1 probe constructs. The cpstFRET module was inserted between amino acids 357 and 358 in the occludin probe and b...