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New algorithm proves to be a powerful tool for detection and identification of novel bacterial organisms

Researchers developed the NOVA algorithm to systematically analyze bacterial isolates unidentified using traditional methods such as matrix-assisted laser desorption ionization-time of flight mass spectrometry and partial 16S rRNA gene sequencing using whole genome sequencing. ....

Kateryna Kon Shutterstock , Bruker Daltonics , University Hospital Basel , National Center , Novel Organism Verification , Biotechnology Information , Prokaryotic Names , Gene Sequencing , Maldi Tof , Mass Spectrometry , Ibonucleic Acid , Whole Genome Sequencing ,

Frontiers | Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties

Common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learning the rhizobial communities associated with common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (Cuican and Jiadouwang) grown in two sites of blackland, and were characterized by comparative sequence analyses of 16S rRNA, recA, atpD, nodC, nifH genes and whole genome. As results, Rhizobium indigoferae, R. anhuiense and R. croatiense as minor groups and three dominant novel Rhizobium species were identified based on their ANI and DDH values to the type strains of relative species. This community composition of rhizobia associated with common bean in the tested black soils was unique. Despite their different species affiliation, all of them were identified into the symbiovar phaseoli according to the phylogenies of symbiotic genes (nodC and nifH). While the discrepancies found in nodC and nifH ....

Inner Mongolia Autonomous Region , Nei Mongol , City Of , United Kingdom , Loess Plateau , China General , Iberian Peninsula , Spain General , United States , South Korea , North China Plain , Africa Mhamdi , Microbiol Spectr , Asia Huo , Guizhou Yeh , Asia Zhang , Europe Rodi , Africa Martin , Sheng Gerald , America Maciel , Europe Garc , Chinese Academy Of Sciences , Institute Of Geography , Agriculture University Harbin City , National Center , Sangon Biotech Shanghai Co Ltd ,