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Queen Letizia, 50 and Jill Biden, 71, both visited Columbia University's new Medical Centre, where they addressed the room ahead of World Cancer Research Day this afternoon.
It is thought the Spanish Queen was unable to attend the Windsor ceremony due to 'scheduling reasons', according to ¡Hola!.
The Queen of Denmark has shared touching throwback photographs of the Queen ahead of the state funeral on Monday. The King and Queen of Sweden also shared a tribute.
The United Kingdom's longest-reigning monarch will be honored by world leaders and mourners around the globe during a service at Westminster Abbey in London.
Ten days of national mourning are coming to a close with the funeral of Britain's Queen Elizabeth II.