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New Nature Communications publication by Mann & Theis Groups harnesses the benefits of large-scale peptide collisional cross section (CCS) measurements and deep learning for 4D-proteomics Bruker Corporation today announces a seminal publication from the groups of Professors Matthias Mann and Fabian Theis in the journal Nature Communications with the title ‘Deep learning the collisional cross sections of the peptide universe from a million experimental values’ by Florian Meier et al. (doi.org/10.1038/s41467-021-21352-8). Figure 1. Large-scale peptide collisional cross section (CCS) measurement with TIMS and PASEF. From Deep learning the collisional cross sections of the peptide universe from a million experimental values . (a) Workflow from extraction of whole-cell proteomes through digestion, fractionation, and chromatographic separation of each fraction. The TIMS-quadrupole TOF mass spectrometer was operated in PASEF mode. (b) Overview of the CCS dataset in this stud ....
Bruker and Evosep announce major progress in unbiased, quantitative true single-cell proteomics Bruker Corporation and Evosep today announced major progress in high-sensitivity, quantitative true single-cell proteomics, using a modified timsTOF Pro mass spectrometer connected to an Evosep One low-flow chromatography system. Evosep One is a chromatography system that delivers fast, sensitive and robust proteomic analysis of clinical samples, while supporting connections to a wide range of mass spectrometers. These advances have been demonstrated by the Mann-group in a paper by A. Brunner et al., which is now available on Bioarchive here. Key results include the first untargeted and unbiased analysis of true single cells achieving an identification depth of up to 1,500 protein groups from single FACS-sorted and individually digested HeLa cells. This remarkable sensitivity was achieved by the combined ....
Press release content from Business Wire. The AP news staff was not involved in its creation. Bruker and Evosep Announce Major Progress in Unbiased, Quantitative True Single-cell Proteomics January 5, 2021 GMT Figure 1: Evosep One LC and timsTOF Pro MS (Photo: Business Wire) BILLERICA, Mass. & ODENSE, Denmark (BUSINESS WIRE) Jan 5, 2021 Bruker Corporation (Nasdaq: BRKR) and Evosep today announced major progress in high-sensitivity, quantitative true single-cell proteomics, using a modified timsTOF Pro mass spectrometer connected to an Evosep One low-flow chromatography system. Figure 1: Evosep One LC and timsTOF Pro MS (Photo: Business Wire) These advances have been demonstrated by the Mann-group in a paper by ....