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Role of LINC00504 in proliferation and invasion of BC.


http://www.mircode.org/) were used to identify the potential binding sequences of miR-140-5p and LINC00504. SKBR3 and MDA-MB-231 cells were seeded in 24-well plates. The cells were cotransfected with miR-140-5p or microRNA NC and luciferase reporter constructs containing WT-LIC00504 or Mut-LINC00504, as previously described.
13 The luciferase activity was detected using a Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime Institute of Biotechnology, Shanghai, China), according to the manufacturer’s protocol. Mutations in the putative binding sites of LINC00504 were made using a Quick-change site-directed mutagenesis kit (200518, Agilent Technologies, Santa Clara, USA). The primers were as follows: LINC00504, Forward primer: 5ʹ-GTGACTCGAGCTTGCCTCTGCCATGT-3ʹ, Reverse primer: 5ʹ-GTGAGCGGCCGCTTTCAGAGTGAAACAATACTT-3ʹ; Mut-LINC00504, Forward primer: 5`-GGATTACAGGCGTGAGGGTGAC-3`; and Reverse primer: 5`-GTCACCCTCACGCCTGTAATCC-3`. ....

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Shakey's to hold annual stockholders' meeting on July 15


BusinessWorld
June 16, 2021 | 7:00 am
Notice of Annual Stockholders’ Meeting
Notice is hereby given that the Annual Stockholders Meeting will be held on Thursday, July 15, 2021 at 8:30 in the morning.
The agenda for the said meeting shall be as follows:
Call to Order
Approval of the Minutes of the Stockholders’ Meeting held on July 15, 2020
Management’s Report
Election of Directors (including Independent Directors)
Appointment of External Auditor
Adjournment
A brief explanation of the agenda items which require stockholders’ approval are provided on the Information Statement. The Information Statement and Annual Report will be uploaded to the Corporation’s website https://www.shakeyspizza.ph/and PSE EDGE. ....

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Century Pacific Food announces annual stockholders' meeting


BusinessWorld
May 28, 2021 | 8:00 am
Notice of Annual Stockholders’ Meeting
Notice is hereby given that the Annual Stockholders’ Meeting will be held on Wednesday, June 30, 2021 at 8:30 in the morning.
The agenda for the said meeting shall be as follows:
1. Call to Order
3. Approval of the Minutes of the Stockholders’ Meeting held on June 30, 2020
4. Management’s Report
6. Election of Directors (including Independent Directors)
7. Appointment of External Auditor
8. Other Matters
9. Adjournment
A brief explanation of the agenda item which requires stockholders’ approval is provided in the Definitive Information Statement. The Definitive Information Statement, Management Report, and Annual Report for 2020 will be uploaded to the Company’s Website at https://www.centurypacific.com.ph/ and at PSE EDGE under Century Pacific Food, Inc. Company Disclosures. ....

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