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Frontiers | Emergence of Dengue 4 as Dominant Serotype During 2017 Outbreak in South India and Associated Cytokine Expression Profile

Dengue virus infection is prevalent in tropical and sub-tropical regions of the world, which is fatal if untreated symptomatically. Emergence of new genotype within serotypes led to enhanced severity. The objective of the study is to identify the molecular characteristics of the DENV circulated during 2017 outbreak in Tamil Nadu, India and, to investigate the role of inflammatory cytokines in different ‘serotypes’ and in ‘dengue severity’. A total of 135 suspected samples were tested for DENV infection using IgM, IgG and qPCR assay; where 76 samples were positive for DENV and analysed for twelve inflammatory cytokines using ELISA. Serotyping shows 14 DENV-1, 22 DENV-2, 7 DENV-3 and 33 DENV-4, where DENV-4 was predominant. Among seventy-six, forty-two isolates were successfully sequenced for C-prM region and grouped. A lineage shift was observed in DENV-4 genotype. Irrespective of serotypes, IFNγ was significantly elevated than control and in primary infection indicating its ro ....

United States , United Kingdom , New Delhi , Tamil Nadu , Sri Lanka , Republic Of , North Western , City Of , Andaman And Nicobar Islands , Saudi Arabia , Gonzalez Mateos , Flores Aguilar , Flavivirus Serocomplex Cross , Vellore Cecilia , Cladec Cecilia , Lakshmana Rao , Alwin Prem Anand , Andrea Wizenmann , Cruz Hernandez , Oswaldo Cruz , Gowri Sankar , Triuppur Alagarasu , Pondicherry Hoti , Waal Malefyt , Lopez Martinez , Nadu Manakkadan ,

Remains of 2,500-Year-Old Mill Discovered in Italy


Remains of 2,500-Year-Old Mill Discovered in Italy
They believe it is the earliest olive oil mill found in Magna Graecia, a region that encompasses most of Italy s southern coastline, where ancient Greek colonists arrived 3,500 years ago.
Photo: Lucano Agency for Development and Innovation in Agriculture
Feb. 12, 2021
An ancient olive oil mill dat­ing to the 4
th cen­tury B.C. has been uncov­ered dur­ing arche­o­log­i­cal exca­va­tions in the province of Matera.
Located in the Basilicata region in south­ern Italy, the archae­ol­o­gists said that the find­ing is sen­sa­tional due to both its struc­ture and age. ....

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Ancient Olive Oil Production Artifact Found in Gethsemane


Ancient Olive Oil Production Artifact Found in Gethsemane
Archaeologists found a 2,000-year-old ritual bath, known as a Mikveh. The finding underscores the importance of olive oil production in Gethsemane during the time of Christ.
Photos: Nadim Asfour.
A 2,000-year-old rit­ual bath used for the pro­duc­tion of olive oil was dis­cov­ered dur­ing exca­va­tions con­ducted in the Gethsemane area, in Jerusalem. The dis­cov­ery was announced dur­ing a press con­fer­ence orga­nized by the Custody of the Holy Land on the site.See Also: New Insights Into the World’s Oldest Bottle of Olive Oil ....

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