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Role of LINC00504 in proliferation and invasion of BC.

http://www.mircode.org/) were used to identify the potential binding sequences of miR-140-5p and LINC00504. SKBR3 and MDA-MB-231 cells were seeded in 24-well plates. The cells were cotransfected with miR-140-5p or microRNA NC and luciferase reporter constructs containing WT-LIC00504 or Mut-LINC00504, as previously described.
13 The luciferase activity was detected using a Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime Institute of Biotechnology, Shanghai, China), according to the manufacturer’s protocol. Mutations in the putative binding sites of LINC00504 were made using a Quick-change site-directed mutagenesis kit (200518, Agilent Technologies, Santa Clara, USA). The primers were as follows: LINC00504, Forward primer: 5ʹ-GTGACTCGAGCTTGCCTCTGCCATGT-3ʹ, Reverse primer: 5ʹ-GTGAGCGGCCGCTTTCAGAGTGAAACAATACTT-3ʹ; Mut-LINC00504, Forward primer: 5`-GGATTACAGGCGTGAGGGTGAC-3`; and Reverse primer: 5`-GTCACCCTCACGCCTGTAATCC-3`. ....

United States , United Kingdom , Baden Wüberg , Grand Island , Eteläuomen Läi , Santa Clara , Hyclone Laboratories Inc , Thermo Fisher , Agilent Technologies , Institute Of Biochemistry , Merck Kga , Merck Kgaa , Beyotime Institute Of Biotechnology , Guangdong Provincial People , Ethics Committee , Hospital Institutional Review Board , Certificate Number , American Type Culture Collection , Cell Biology , Chinese Academy , Life Technologies , Hyclone Laboratories , Applied Biosystems , Foster City , Immunoprecipitation Assay , Zyme Chromatin Immunoprecipitation Kit ,

The impact of COVID-19 on maternity care - Trinity Research

The impact of COVID-19 on maternity care
To explore the impact of COVID-19 on maternity care, Prof. Valerie Smith and colleagues in the School of Nursing and Midwifery evaluated both clinical and experiential outcomes in a two-phase study at one hospital site in Ireland.
Prof. Valerie Smith, Dr Sunita Panda, Dr Deirdre O’Malley: School of Nursing and Midwifery, TCD
Ms Paula Barry, Ms Nora Vallejo: Coombe Women and Infants University Hospital
Funding: Trinity College Dublin’s Office of the Dean of Research COVID-19 Response Fund
Background
In March of 2020, the first of a series of national lockdowns was implemented in Ireland as a means of reducing the community spread of COVID-19. This resulted in changes to maternity care practices that had the potential to impact on the clinical and experiential outcomes of women accessing maternity care during this time. Some of these changes included policies of restrictive visiting (e.g. one designated p ....

Paula Barry , Deirdre Omalley , Valerie Smith , Nora Vallejo , During Group , Infants University Hospital , Hospital Research Ethics , School Of Nursing , Response Fund , Coombe Women , Infants University , Trinity College , Hospital Research , Trinity College Dublin , University Of Dublin , Post Graduate , பால பாரி , டெறின்றே ஓமல்லேய் , வலேரி ஸ்மித் , நோரா வாலெஜோ , போது குழு , கைக்குழந்தைகள் பல்கலைக்கழகம் மருத்துவமனை , மருத்துவமனை ஆராய்ச்சி நெறிமுறைகள் , பள்ளி ஆஃப் நர்சிங் , பதில் நிதி , கூம்பே பெண்கள் ,