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Virucidal activity of antiseptic mouthwash and dental gel

Negative Control: Cellular system only
Treatment 1: Cellular system, Viral solution and Dental Gel APD (dilution without cytotoxic effect)
Treatment 2: Cellular system, Viral solution and Mouthwash APD (dilution without cytotoxic effect)
The determination of the virucidal activity of the product is carried out according to the Methodology TCID
50 (Median Tissue Culture Infectious Dose). The protocol considers the cultivation of the host cell lines of the virus – Vero ATCC cells
® CCL-81™ in culture microplates of 96 wells with subsequent dilution of the viral solution and the possible virucidal compound to be analyzed.
To evaluate the cytopathic effect, analyses are performed by means of an inverted microscope after an incubation period of 72 hours in an oven at 37°C with 5% CO ....

Spearman Karber Lorenz , Oral Health Technologies , Angiotensin Converting Enzyme , Modified Eagle , Maximum Non Cytotoxic Concentration , Dental Gel , Oral Health , Effect Test , Product Contact , Median Tissue Culture Infectious Dose , Negative Control , Titer After Exposure , Viral Inactivation , மாற்றப்பட்டது கழுகு , டெஂடல் கேள் , வாய்வழி ஆரோக்கியம் , ப்ராடக்ட் தொடர்பு , எதிர்மறை கட்டுப்பாடு ,

CircHIPK3 involvement in Breast Cancer via MiR-326

2
1Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China;
2The 2nd Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People’s Republic of China
Correspondence: Liqiang Qi
Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 17, Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People’s Republic of China
Tel +86-10-67781331
Purpose: circHIPK3 has carcinogenic or anti-tumor effects on different cancers. However, there is no relevant research showing whether circHIPK3 was involved in breast cancer (BCa). In this research, the aim was to analyze the function and possible molecular mechanism of circHIPK3 in BCa. ....

United States , United Kingdom , Eteläuomen Läi , Union Medical College , Co Ltd , Information Technology Center , National Institutes Of Health , Cancer Institute , Ethics Committee Of Cancer Institute , China Council On Animal Care , Beckman Coulter , Committee Of Cancer Institute , Chinese Academy Of Medical Sciences , Cytoplasmic Nuclear , Cancer Hospital , Chinese Academy , Medical Sciences , Peking Union Medical College , Ethics Committee , Institutional Animal Care , Use Committee , China Council , Animal Care , Modified Eagle , Fetal Bovine Serum , American Type Culture Collection ,

Preparation, Synergism, and Biocompatibility of in situ Liquid Crystal

Preparation, Synergism, and Biocompatibility of in situ Liquid Crystals Loaded with Sinomenine and 5-Fluorouracil for Treatment of Liver Cancer ....

United States , New Zealand , Sprague Dawley , Group Of The Same Culture Time , Shanghai Xinyi Jinzhu Pharmaceutical Co Ltd , Anhui Medical University Hefei , Laboratory Animals , Shanghai Ika Biotechnology Co Ltd , Sinopharm Chemical Reagent Co Ltd , Shanghai Targetmol Biotechnology Co Ltd , Software Including , Tokyo Chemical Industry Co Ltd , Hemolysis Experiment Group Design , Chengdu Must Biotechnology Co Ltd , Technology Co Ltd , Experimental Animal Center , Anhui Medical University , Animal Experiments , Anhui University , Chinese Medicine , National Institutes , Health Guide , Red Blood Cell , Experiment Group , Modified Eagle , Various Different Dilution Ratios ,

Vascular Smooth Muscle Cells via JNK/c-JUN Pathway

Purpose: The deposition of hydroxyapatite (HAp) crystals plays an important role in the development of vascular calcification (VC). This study aimed to demonstrate the effects of nanosized HAp (nHAp) on vascular smooth muscle cells (VSMCs) and VC progression.
Methods: Transmission electron microscopy (TEM) was used to examine cellular uptake of nHAp. Cell viability was determined using CCK-8 assay kit. Mitochondrial impairment and reactive oxygen species were detected by TEM and fluorescence dye staining, respectively. Cell apoptosis was detected by Western blot analysis and Annexin V staining. Mouse model of VC was built via applying nHAp on the surface of abdominal aorta. Calcification was visualized by Alizarin red and von Kossa staining. ....

Minato Ku , United States , United Kingdom , Hexadecyl Pyridinium , Annexinv Annexin , Laboratory Animals , United States National Institutes Of Health , Program Graph , Second Affiliated Hospital Of Zhejiang University , Alizarin Red Solarbio Life Sciences , Slac Laboratory Animal , Ethics Review Committee , Aladdin Biochemical Technology Company Shanghai , Care Committee Of Zhejiang University , Cell Signaling Technology , Kit Solarbio Life Sciences , Modified Eagle , Aladdin Biochemical Technology Company , Cell Counting , Beyotime Biotechnology , Reactive Oxygen Species , Electron Microscopy , Hitachi Model , Mitochondrial Membrane Potential , Alkaline Phosphatase , San Jose ,

The roles of circRNAs in neuroblastoma

CircRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: selected key circRNAs in neuroblastoma and their associations with clinical features ....

United States , Institute Research Ethics Committee , Graphpad Software Inc , Children Hospital Of Fudan University Shanghai , Chinese Academy Of Science Shanghai , Cell Bank , Fudan University , Five Paired Neuroblastoma Samples Used , Life Technologies , Kyoto Encyclopedia , Thermo Scientific , Chinese Academy , Modified Eagle , Nutrient Mixture , Cell Counting , Dojindo Laboratories , Graphpad Prism , Graphpad Software , Selected Differentially Expressed , Between Significantly Downregulated , Fold Decreases Among , Clinical Features , Favorable Histology , ஒன்றுபட்டது மாநிலங்களில் , சீன கலைக்கழகம் ஆஃப் அறிவியல் ஷாங்காய் , செல் வங்கி ,