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New isolated lymphatic vessel lumen perfusion system to evaluate nanoparticle dynamics Nanoparticles used in drug delivery systems, bioimaging, and regenerative medicine migrate from tissues to lymphatic vessels after entering the body, so it is necessary to clarify the interaction between nanoparticles and lymphatic vessels. Although technology to observe the flow of nanoparticles through lymphatic vessels in vivo has been developed, there has been no method to evaluate the flow of nanoparticles in a more detailed and quantitative manner ex vivo. Thus, research was conducted to develop an ex vivo lymphatic vessel lumen perfusion system to determine how nanoparticles move in lymphatic vessels and how they affect the physiological movement of lymphatic vessels. ....