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Improving prime editing with an endogenous small RNA-binding protein

Prime editing enables the precise modification of genomes through reverse transcription of template sequences appended to the 3′ ends of CRISPR–Cas guide RNAs1. To identify cellular determinants of prime editing, we developed scalable prime editing reporters and performed genome-scale CRISPR-interference screens. From these screens, a single factor emerged as the strongest mediator of prime editing: the small RNA-binding exonuclease protection factor La. Further investigation revealed that La promotes prime editing across approaches (PE2, PE3, PE4 and PE5), edit types (substitutions, insertions and deletions), endogenous loci and cell types but has no consistent effect on genome-editing approaches that rely on standard, unextended guide RNAs. Previous work has shown that La binds polyuridine tracts at the 3′ ends of RNA polymerase III transcripts2. We found that La functionally interacts with the 3′ ends of polyuridylated prime editing gu ....

Staphylococcusaureus Cas , Acids Res , Protein Cell , Open Source Softw ,

Lady Bulldogs maul Lady Warriors, assure of at least a playoff berth

Lady Bulldogs maul Lady Warriors, assure of at least a playoff berth
mb.com.ph - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from mb.com.ph Daily Mail and Mail on Sunday newspapers.

Santo Tomas , Steven Aligayon , Spiker Michaelo Buddin , Mhicaela Belen , Casiey Dongallo , Bella Belen , Leo Aringo , University Of Santo Tomas Golden Tigresses , University Of The , National University , Final Four , Asia Arena , Lady Bulldogs , Santo Tomas Golden Tigresses , Holy Week , Alyssa Solomon , Lady Warriors , Best Outside Spiker Michaelo Buddin , Red Warriors ,

NU's Belen tests resolve of UE rookie sensation; Poyos, UST eye big rebound – Tempo – The Nation's Fastest Growing Newspaper

By NIKOLE JAVIER League-leading University of Santo Tomas is looking to pick up itself when it goes up ....

Santo Tomas , Angge Poyos , Casiey Dongallo , Bella Belen , University Of Santo Tomas , National University Lady Bulldogs , University Of The , Asia Arena , Golden Tigresses , Lady Falcons , Holy Week , Lady Bulldogs , Lady Warriors , Valuable Player Bella Belen ,

Port of Baltimore Disaster: Automotive & Supply Chain Perspective

Port of Baltimore Disaster: Automotive & Supply Chain Perspective
automotive-fleet.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from automotive-fleet.com Daily Mail and Mail on Sunday newspapers.

New York , United States , New Jersey , Brice Adamson , Rick Franza , Hull College Of Business At Augusta University , Land Rover , Jaguar Land Rover , Home Depot , Automotive Fleet , Francis Scott Key Bridge , Costa Concordia , Gulf Coast , East Coast , Hull College , Augusta University , General Motors , Enterprise Fleet ,

No. 3 Syracuse dominates Cornell 17-4, wins 7th straight game

In its final nonconference game of the season, No. 3 Syracuse allowed a season-low four goals in a blowout win over Cornell. ....

Julia Basciano , Emma Tyrrell , Nellie Horner , Emma Muchnick , Savannah Sweitzer , Maddy Baxter , Payton Rowley , Emma Tyrell , Josie Vogel , Olivia Adamson , Natalie Smith , Kate Mashewske , Mckenzie Olsen , Delaney Sweitzer , Kayla Treanor , Katie Goodale , Ivy League , Coast Conference , Big Red , Meghan Rode ,