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Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage

Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage
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Performance characteristics of the RNA and the high sensitivity RNA ScreenTape assays for the 4150 TapeStation System

The Agilent 4150 TapeStation system is an automated electrophoresis solution for fast and reliable nucleic acid sample quality control. The entire Agilent RNA and DNA ScreenTape portfolio is applicable on the 4150 TapeStation system. The Agilent RNA ScreenTape assays enable separation, integrity, and quantity analysis of total RNA samples from both eukaryotic and prokaryotic origin. The RNA integrity number equivalent (RIN ....

Agilent Technologies , Application Note , Agilent High Sensitivity , Agilent 4150 ,

Next-Generation Sequencing and Bioinformatics-Based Protocol for the F

Next-Generation Sequencing and Bioinformatics-Based Protocol for the Full-Length CYP2E1 Gene Polymorphism Analysis ....

Eteläuomen Läi , United States , Santa Clara , Agilent Technologies , Centre Of Tuberculosis , Ethical Committee Of Riga Stradins University , Clinical Pharmacogenetics Implementation Consortium , Riga East University Hospital , Ethical Committee Of Riga East University Hospital , Genome Database , Helsinki Declaration , Central Medical Ethics , Ethical Committee , Riga Stradins University , All Taq , Size Select , Life Technologies , Nextera Index , Agilent High Sensitivity , Miseq Reagent Nano Kit , Brilliantdye Terminator Cycle Sequencing , Logical Alignment Search Tool , Data Quality , Study Sample Set , Thermo Fisher Scientific Baltics , Identified Single Nucleotide ,

Top tips for RNA-sequencing that involves degraded inputs

In this article, Roche Diagnostics elucidates upon some of their top tips and recommendations for anyone performing RNA-sequencing with degraded RNA inputs and demonstrate how to improve efficiency and performance. ....

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Epigenomic landscape and 3D genome structure in pediatric high-grade glioma

Pediatric high-grade gliomas (pHGGs), including glioblastoma multiforme (GBM) and diffuse intrinsic pontine glioma (DIPG), are morbid brain tumors. Even with treatment survival is poor, making pHGG the number one cause of cancer death in children. Up to 80% of DIPGs harbor a somatic missense mutation in genes encoding histone H3. To investigate whether H3K27M is associated with distinct chromatin structure that alters transcription regulation, we generated the first high-resolution Hi-C maps of pHGG cell lines and tumor tissue. By integrating transcriptome (RNA-seq), enhancer landscape (ChIP-seq), genome structure (Hi-C), and chromatin accessibility (ATAC-seq) datasets from H3K27M and wild-type specimens, we identified tumor-specific enhancers and regulatory networks for known oncogenes. We identified genomic structural variations that lead to potential enhancer hijacking and gene coamplification, including A2M , JAG2 , and FLRT1 . Together, our results imply three-dimensional genome a ....

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