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A community approach for pathogens and their arthropod vectors (ticks and fleas) in cats of sub-Saharan Africa | Parasites & Vectors

Arthropod-borne pathogens and their vectors are present throughout Africa. They have been well studied in livestock of sub-Saharan Africa, but poorly studied in companion animals. Given their socioeconomic importance, the African Small Companion Animal Network (AFSCAN), as part of the WSAVA Foundation, initiated a standardized multi-country surveillance study. In six countries (Ghana, Kenya, Nigeria, Tanzania, Uganda, and Namibia) in both rural and urban settings, 160 infested cats were sampled to assess their ectoparasite community (ticks and fleas), as well as the micro-parasite prevalence within those ectoparasites (60 and 118 pools of ticks and fleas, respectively) and blood (276 cats, including 116 non-infested). Almost two thirds of all infested cats originated from Tanzania and Kenya. Despite the large macro-geographical variation, no consistent difference was found in ectoparasite diversity and numbers between East and West Africa. Far more flea-infested than tick-infested cats ....

Ghana General , United States , Nairobi Area , South Africa , Mbarara District , Rift Valley , Ashanti Region , Greater Accra Region , Greater Accra , Atlantic Ocean , Chelex Bio Rad , East Legon , North Central , Dar Es Salaam , Plus Test , Universal Probe , New England Biolabs , Hot Start High Fidelity , Master Mix , Basic Local Alignment Search Tool ,

Establish an allele-specific real-time PCR for Leishmania species identification | Infectious Diseases of Poverty

Leishmaniasis is a serious neglected tropical disease that may lead to life-threatening outcome, which species are closely related to clinical diagnosis and patient management. The current Leishmania species determination method is not appropriate for clinical application. New Leishmania species identification tool is needed using clinical samples directly without isolation and cultivation of parasites. A probe-based allele-specific real-time PCR assay was established for Leishmania species identification between Leishmania donovani and L. infantum for visceral leishmaniasis (VL) and among L. major, L. tropica and L. donovani/L. infantum for cutaneous leishmaniasis (CL), targeting hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and spermidine synthase (SPDSYN) gene with their species-specific single nucleotide polymorphisms (SNPs). The limit of detection of this assay was evaluated based on 8 repeated tests with intra-assay standard deviation < 0.5 and inter- ....

United States , New Zealand , Sangon Biotech Co Ltd , Capital Medical University , Beijing Friendship Hospital , Universidad Nacional De Salta Argentina , Ibis Biosciences , Applied Biosystems Roche , Basic Local Alignment Search Tool , Tissue Kit , Promega Gotaq Probe , Applied Biosystems , Mega Limited , Neighbor Joining ,