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Long non-coding RNA Duxap8 facilitates cell proliferation

Long non-coding RNA Duxap8 facilitates cell proliferation
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South Korea , United States , Eteläuomen Läi , Baden Wüberg , Trizol Invitrogen , Santa Cruz Inc , Humane Treatment Of Laboratory Animals , Beyotime Inc , Institutional Research Committee , Millipore Inc , Shanghai Experimental Animal Centre , Cell Bank , Cell Signaling Technology , First Affiliated Hospital Of Soochow University , Institutional Research Ethics Committee , Chinese Academy Of Science Shanghai , Standards For Laboratory Animals , Beyotime Institute Of Biotechnology , First Affiliated Hospital , Soochow University , Chinese Academy , Cell Counting , Beyotime Institute , Flow Cytometry , Binding Protein Immunoprecipitation Kit , Eosin Staining ,

GARP and GARP-treated tDC prevented atherosclerotic plaques

GARP and GARP-treated tDC prevented atherosclerotic plaques
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United States , Co Ltd , Protein Assay Kit Beyotime Institute Of Biotechnology , Body Weight In Each Group , T Cells Were Co , Mp Biomedicals Co Ltd , Laboratory Animals Science , Ethical Committee Of Huazhong University Science , Aldrich Inc , Technology Department Of Hubei Province , Ethical Committee , Huazhong University , Laboratory Animals , Technology Department , Hubei Province , Beach Group , Masson Trichrome Stain Kit , Sigma Aldrich Inc , Grand Island , Different Types , Assay Kit , Beyotime Institute , Santa Cruz , Blotting Detection Reagents , Bio Rad Laboratories , Linked Immunosorbent Assay ,

Role of LINC00504 in proliferation and invasion of BC.

http://www.mircode.org/) were used to identify the potential binding sequences of miR-140-5p and LINC00504. SKBR3 and MDA-MB-231 cells were seeded in 24-well plates. The cells were cotransfected with miR-140-5p or microRNA NC and luciferase reporter constructs containing WT-LIC00504 or Mut-LINC00504, as previously described.
13 The luciferase activity was detected using a Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime Institute of Biotechnology, Shanghai, China), according to the manufacturer’s protocol. Mutations in the putative binding sites of LINC00504 were made using a Quick-change site-directed mutagenesis kit (200518, Agilent Technologies, Santa Clara, USA). The primers were as follows: LINC00504, Forward primer: 5ʹ-GTGACTCGAGCTTGCCTCTGCCATGT-3ʹ, Reverse primer: 5ʹ-GTGAGCGGCCGCTTTCAGAGTGAAACAATACTT-3ʹ; Mut-LINC00504, Forward primer: 5`-GGATTACAGGCGTGAGGGTGAC-3`; and Reverse primer: 5`-GTCACCCTCACGCCTGTAATCC-3`. ....

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MiR-126a-5p in regulating NOX2 in ischemic stroke

MicroRNA-126a-5p exerts neuroprotective effects on ischemic stroke via targeting NADPH oxidase 2 ....

United States , T Ai Pei , United Kingdom , Supermix Transgen , Shanghai Genmed Pharmaceutical Technology Co Ltd , University Of Science , Chinese Academy Of Sciences Shanghai , Laboratory Animal Center , Cell Bank , Pierce Company , Nanchang University , Laboratory Animal Safety , Ethics Committee Of The Institutional Animal Care , Beyotime Institute Of Biotechnology , Shanghai Institute Of Cell Biology , Committee Of Nanchang University , Sigma Aldrich , System Bio Rad Co , Technology Of China , Ethics Committee , Institutional Animal Care , Use Committee , Shanghai Institute , Cell Biology , Chinese Academy , American Type Culture Collection ,

Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Develo

Depletion of Circular RNA circ CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis ....

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