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Arrested earlier this month, members of the group claim they own the land as heirs of Native Hawaiians who got land grants during the Great Mahele in 1848.
The squatters had taken over the private property nine months ago claiming they had title under laws of the Hawaiian Kingdom.
A circuit judge ruled in favor of the property owners and signed an order directing police or sheriffs to remove the defendants and their supporters.
The owners of the 5-acre site intended to grow medical marijuana have a court order to remove the activists who are refusing to leave.