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Researchers demonstrate targeted epigenome editing in the promoter region of several genes using sgRNA/dCas9 complexes

Researchers develop and characterize a highly specific EpiEditing system using catalytically inactivated catalytically inactivated Cas9. ....

Cgis Cp , Research Square , Golden Gate Assembly , Golden Gate , Na Methylation , Ene Expression , Ibonucleic Acid ,

"Expression and role of CoREST3 in neurodevelopment and neurodegenerati" by Simon Maksour

Repressor element-1 silencing transcription factor (REST) is a transcriptional repressor involved in neurodevelopment and neuroprotection that forms a complex with the corepressor of REST1 (CoREST1), CoREST2 and CoREST3 (encoded by RCOR1, RCOR2 and RCOR3, respectively). Emerging evidence suggests the CoREST family have the ability to target unique genes, in a RESTindependent manner, in various neural and glial cell types at different stages of development. There is limited knowledge on expression and function of CoREST3 in neurodevelopment and neurodegeneration, particularly in humans. This study used 2D and 3D human pluripotent stem cell (hPSC) models to interrogate RCOR3 gene expression levels in human neurons using RT-qPCR. RCOR3 expression was shown to significantly increase in glutamatergic cortical and GABAergic ventral forebrain neurons, mature functional NGN2 induced neurons and a trend for an increase in 3D cerebral organoids. CoREST3 was shown to have an age-related and regio ....

Alzheimers Disease , Stem Cells ,

Moderate exercise helps fight inflammation at the cellular level

Researchers investigated the effects of moderate exercise on inflammation and found that it reduced inflammatory markers. This was due to epigenetic changes that affected the expression of genes responsible for the inflammatory responses of certain immune cells. ....

United States , Tejasav Sehrawat , Ryan Glatt , Ali Abdul Sater , Jacob Teitelbaum , Isabelle Amigues , York University , Yale University School Of Medicine , Medical News Today , Yale University School , Mayo Clinic , Fitbrain Program , Pacific Neuroscience Institute , Santa Monica ,