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tiRNA-Val-C Fragment Inhibit Gastric Cancer Cells by LRP6

Investigating the potential biological function and molecular mechanism of tsRNA in GC cells preliminary. Click to read more.. ....

United States , France General , Gibco Invitrogen , Cancer Hospital Of Nanjing Medical University , Technology Company Shanghai , East Asia , Affiliated Cancer Hospital , Nanjing Medical University , Clinical Research Ethics Committee , Biological Science , Technology Company , National Collection , Keygen Biotech , Quantitative Polymerase Chain Reaction , Life Technologies , Primers Used , Molecular Devices , Apoptosis Reagent , Cytoplasm Separation , Loading Buffer , Abcam United States , Affinity United States , Bio Imaging Systems , Kyoto Encyclopedia , Graphpad Prism , Wnt Signaling ,

The diagnostic and prognostic value of hsa_circ_0084927

Analyzing the differentially expressed circRNA in colon adenocarcinoma and evaluating its diagnostic and prognostic value ....

Eteläuomen Läi , United States , Circrna Mirna Mrna Network Prediction , Guangxi Medical University Cancer Hospital , Transwell Chamber Experiments Detect Cell Invasion , Transwell Chamber Experiments Detect Cell Migration , Ethics Committee , American Type Culture Collection , Takara Japan , Faststart Universal , Guangzhou Ruibo , Detect Cell , Transwell Chamber Experiments Detect Cell , Protein Interaction , Hub Gene Construction , Molecular Complex Detection , Set Enrichment Analysis , Clinical Characteristics , Clinicopathological Parameters , Expression Level , Its Baseline , Hub Genes , Human Protein Altas Database , Baseline Features , Human Protein Atlas ,

tRNA-Derived Fragments in Hypopharyngeal Carcinoma

2
1Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan Province, 410008, People’s Republic of China;
2Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, Hunan, People’s Republic of China;
3Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan Province, 410008, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Jing Xu
Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan Province, 410008, People’s Republic of China
Objective: To detect the expression level of tRNA-derived fragments secreted by extracellular vesicles in hypopharyngeal cancer and explore the influence of tRNA-derived fragments on the occurrence of hypopharyngeal cancer and lung metastasis. ....

United States , Eteläuomen Läi , Department Of Otolaryngology , Neck Surgery , American Type Culture Collection , Scanning Electron Microscopy , Extracellular Vesicles , Illumina Nextseq , Basic Informations , Quantitative Real Time , Primer Sequence , Hypopharyngeal Cancer Patients , Clinical Characteristics , Hypopharyngeal Cancer , Analysis Between , Expression Level , Its Clinicopathological Characteristics , Newly Treated , Were Closely Related , Relationship Between , Clinicopathological Characteristics , Regression Analysis , Risk Factors , Multivariate Cox Regression Analysis , ஒன்றுபட்டது மாநிலங்களில் , துறை ஆஃப் ஓதோழாரய்ங்கொலொகி ,

[Full text] Circ_0006168 promotes ESCC cell proliferation and mobility

Cell Lines
ESCC cell lines (Eca109, KYSE150, KYSE180 and KYSE450) and human esophageal epithelial cell line HET-1A were purchased from the Shanghai Institute of Chinese Academy of Sciences Cell Collection (Shanghai, China). All these five cell lines were cultured with Roswell Park Memorial Institute-1640 medium (RPMI-1460; Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco), 1% 100 units/mL penicillin (Gibco) and 1% 100 μg/mL streptomycin (Gibco). All cell lines were maintained at 37°C incubator with 5% CO
2.
Western Blot Assay
ESCC cells were collected and lysed using whole cell lysis buffer (Abcam). Protein quantification was conducted with the commercial bicinchoninic acid (BCA) protein assay kit (Invitrogen, Carlsbad, CA, USA). Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred onto the polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA). After block ....

United States , United Kingdom , Eteläuomen Läi , Rnaser Geneseed , Laboratory Animals , Vital River Laboratory Animal Technology , National Guide For The Care , First Affiliated Hospital Of Nanchang University , First Affiliated Hospital , Medical Ethics Committee , Nanchang University , Between Clinicopathological Features , Expression Level , Shanghai Institute , Chinese Academy , Sciences Cell Collection , Roswell Park Memorial , Amersham Pharmacia Biotech , Little Chalfont , Sangon Biotech , San Jose , Diphenyltetrazolium Bromide , Institutional Animal Care , Use Committee , National Guide , Time Quantitative Polymerase Chain Reaction ,

[Full text] Decitabine and Cisplatin are Synergistic to Exert Anti-Tumor Effect on

F) normal.
P 0.05, Compared with moderately differentiated and poorly differentiated tissues, Sox2 mRNA was significantly higher in well-differentiated tissues.
The Association of Sox2 Expression with Clinicopathological Parameters
To further investigate the association between Sox2 expression and clinicopathological parameters. 60 samples were divided into two groups according to Sox2 staining intensity, including high Sox2 staining (n 19) and low Sox2 staining (n 41). We found the Sox2 expression had a strong association with depth of invasion (0.0011), lymph node metastasis (0.0013) and TNM stage (0.0002). However, Sox2 expression had no significant correlation with age (0.4311 and gender (0.8960) (Table 3).
Table 3 Correlation of Sox2 Expression with Clinicopathological Characteristics
DNA Methyltransferase Inhibitor Can Inhibit the Growth, Migration and Invasion of BGC-823 Cell Lines ....

Revertra Ace , Xiamen University Laboratory Animal Center , First Affiliated Hospital Of Xiamen University , Department Of Cancer Center , Thermo Fisher Scientific Inc , Department Of Gastrointestinal Surgery , First Affiliated Of Xiamen University , Tissue Samples , Cell Lines , Gastrointestinal Surgery , First Affiliated Hospital , Xiamen University , Ethics Committee , Cancer Center , First Affiliated , Xiamen University Laboratory Animal , Thermo Fisher Scientific , Revertra Aceh , Aza Cdr Inhibited Transplanted Tumors , Nude Mice , Different Drug Treatment , Transplanted Tumors , Significantly Clinicopathological , Different Differentiated Gastric , Surrounding Nontumor , Different Differentiated Gastric Tissues ,