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Investigating the potential biological function and molecular mechanism of tsRNA in GC cells preliminary. Click to read more..

Analyzing the differentially expressed circRNA in colon adenocarcinoma and evaluating its diagnostic and prognostic value

2 1Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan Province, 410008, People’s Republic of China; 2Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, Hunan, People’s Republic of China; 3Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan Province, 410008, People’s Republic of China *These authors contributed equally to this work Correspondence: Jing Xu Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South Univ...

Cell Lines ESCC cell lines (Eca109, KYSE150, KYSE180 and KYSE450) and human esophageal epithelial cell line HET-1A were purchased from the Shanghai Institute of Chinese Academy of Sciences Cell Collection (Shanghai, China). All these five cell lines were cultured with Roswell Park Memorial Institute-1640 medium (RPMI-1460; Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco), 1% 100 units/mL penicillin (Gibco) and 1% 100 μg/mL streptomycin (Gibco). All cell lines were maintained at 37°C incubator with 5% CO 2. Western Blot Assay ESCC cells were collected and lysed using ...

F) normal. * P 0.05, Compared with moderately differentiated and poorly differentiated tissues, Sox2 mRNA was significantly higher in well-differentiated tissues. The Association of Sox2 Expression with Clinicopathological Parameters To further investigate the association between Sox2 expression and clinicopathological parameters. 60 samples were divided into two groups according to Sox2 staining intensity, including high Sox2 staining (n 19) and low Sox2 staining (n 41). We found the Sox2 expression had a strong association with depth of invasion (0.0011), lymph node metastasis (0.0013) and ...