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Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease

The specific loss of midbrain dopamine neurons (mDANs) causes major motor dysfunction in Parkinson’s disease, which makes cell replacement a promising therapeutic approach1–4. However, poor survival of grafted mDANs remains an obstacle to successful clinical outcomes5–8. Here we show that the surgical procedure itself (referred to here as ‘needle trauma’) triggers a profound host response that is characterized by acute neuroinflammation, robust infiltration of peripheral immune cells and brain cell death. When midbrain dopamine (mDA) cells derived from human induced pluripotent stem (iPS) cells were transplanted into the rodent striatum, less than 10% of implanted tyrosine hydroxylase (TH)+ mDANs survived at two weeks after transplantation. By contrast, TH− grafted cells mostly survived. Notably, transplantation of autologous regulatory T (Treg) cells greatly modified the response to needle trauma, suppressing acute neuroinflammation and immune cell ....

J Neuroimmune Pharmacol , Stem Cell , Cell Res , Cell Rep , Neuroimmune Pharmacol ,

Frontiers | The Musculoskeletal Involvement After Mild to Moderate COVID-19 Infection

COVID-19, a disease caused by the novel coronavirus SARS-CoV-2, has been drastically affecting the daily lives of millions of people. COVID-19 is described as a multiorgan disease that affects not only the respiratory tract of infected individuals, but it has considerable effects on the musculoskeletal system, causing excessive fatigue, myalgia, arthralgia, muscle weakness and skeletal muscle damage. These symptoms can persist for months, decreasing the quality of life of numerous individuals. Curiously, most studies in the scientific literature focus on patients who were hospitalized due to SARS-CoV-2 infection and little is known about the mechanism of action of COVID-19 on skeletal muscles, especially of individuals who had the mild to moderate forms of the disease (non-hospitalized patients). In this review, we focus on the current knowledge about the musculoskeletal system in COVID-19, highlighting the lack of researches investigating the mild to moderate cases of infection and po ....

Pan American Health Organization , District Of Columbia , United States , United Kingdom , City Of , National Institutes Of Health , The Bench , Faroe Islands , Treasure Island , Bangladesh General , Daei Sorkhabi , J Neuroimmune Pharmacol , Le Turnier , Carcamo Garcia , Campos Florian , Salazar Linares , Afamefuna Victor , Gourna Paleoudis , El Sahly , Van Herck , Al Jody , Van Laethem , Acta Myol , Garcia Choza , Alberti Minutti , Abu Hanoud ,

Frontiers | Molecular Docking and Dynamics Studies to Explore Effective Inhibitory Peptides Against the Spike Receptor Binding Domain of SARS-CoV-2

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a pandemic due to the high transmission and mortality rate of this virus. The world health and economic sectors have been severely affected by this deadly virus, exacerbated by the lack of sufficient efficient vaccines. The design of effective drug candidates and their rapid development is necessary to combat this virus. In this study, we selected 23 antimicrobial peptides from the literature and predicted their structure using PEP-FOLD 3.5. In addition, we docked them to the SARS-CoV-2 spike protein receptor-binding domain (RBD) to study their capability to inhibit the RBD, which plays a significant role in virus binding, fusion and entry into the host cell. We used several docking programs including HDOCK, HPEPDOCK, ClusPro, and HawkDock to calculate the binding energy of the protein-peptide complexes. We identified four peptides with high binding free energy and docking scores. The docking results ....

Saudi Arabia , United States , San Diego , J Neuroimmune Pharmacol , Albiol Matanic , Dermatophagoides Pteronyssinus , Silico Pharmacol , Van Doremalen , Raihan Uddin , Acta Tropica , Toxinpred Gupta , Binding Free Energy Calculation , Accelrys Inc , Institute Of India , Taif University , Protein Docking Energy , Free Energy Decomposition For The Ras , Protein Data Bank , Taif University Researchers , Amber Force Field , State Institute , Hidden Markov Model , Protein Data , Discovery Studio , Particle Mesh Ewald , Supplementary Figure ,