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Melatonin Alleviates Age-Associated Endothelial Injury of Atherosclero

Melatonin Alleviates Age-Associated Endothelial Injury of Atherosclerosis via Regulating Telomere Function ....

United States , Assay Kit Beijing Baiaolaibo Technology Co , Jiancheng Bioengineering Institute , Model Animal Research Center , Merck Kga , Cell Signaling Technology , Merck Kgaa , Ethics Committee Of The Nanjing First Hospital , Fude Biological Technology , Beyotime Institute Of Biotechnology , Nanjing University , Ethics Committee , Nanjing First , Apoptosis Detection Kit , Keygen Biotech , Igg Alexa Fluor , Enzyme Linked Biotechnology , Cayman Chemical , Ann Arbor , Nanjing Jiancheng Bioengineering Institute , Frozen Section Reactive Oxygen Species , Assay Kit , Thermo Fisher Scientific , Roche Diagnostics Gmbh , American Type Culture Collection , South Logan ,

BSA NPs of naproxen platinum(IV) have antitumor efficacy

BSA NPs of naproxen platinum(IV) have antitumor efficacy
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United States , United Kingdom , Zetasizer Nano , Tumor Target Properties , Boyuan Chemical Co , Procell Life Science Technology Co Ltd China , Ltd Jinan , Jiancheng Bioengineering Institute , Laboratory Animal Center , Jk Scientific Ltd , Shandong University , Boyuan Chemical , Procell Life Science , Cellular Cytotoxicity , Graphpad Prism , Institute Animal Ethics Committee , Liaocheng University , Nanjing Jiancheng Bioengineering Institute , Tecan Spark , Whole Cells , Preparation Kit , Spectra Assays , Naproxen Platinum , Particle Size , Zeta Potential , Complexes Toward Four Carcinoma Cell Lines ,

Lemon Peel Extract on Oxidative Stress in Rat Heart Cells

Statistical Analysis
Three parallel experiments were performed to obtain the average value, which was determined using SPSS 23 statistical software and compared between groups using independent-sample t tests and a one-way analysis of variance method. Significant differences between groups were observed at the
P 0.05 level.
DPPH and ABTS Radical Scavenging Abilities of LPE
Figure 1 shows the dose-response relationship of DPPH and the ABTS radical scavenging ability of LPE. The ability of LPE to scavenge DPPH and ABTS radicals increased with increasing concentrations of LPE from 0 to 120 μg/mL. Moreover, the Vc positive control group had lower DPPH and ABTS radical scavenging abilities than LPE. ....

United States , Santa Clara , Shanghai Yiji Biology Co Ltd , Jiancheng Bioengineering Institute , Procell Life Science Technology Co Ltd , Sichuan Province , Performance Liquid Chromatographic Detection , Radical Scavenging Ability , Procell Life Science , Cell Viability Using , Thermo Fisher Scientific , Nanjing Jiancheng Bioengineering Institute , Stepone Plus , Oxidative Damage Induced , Lemon Peel Extract , ஒன்றுபட்டது மாநிலங்களில் , சாந்தா கிளாரா , ஸிச்வாந் மாகாணம் , ப்ரோசேல்ல் வாழ்க்கை அறிவியல் , தெர்மோ மீனவர் அறிவியல் , எலுமிச்சை தலாம் பிரித்தெடுத்தல் ,

Punicalagin may be a potent drug for rheumatoid arthritis

1
1Department of Nephrology, Kidney and Urology Center, The Seventh Affiliated Hospital of Sun Yat-Sen University, ShenZhen, Guandong, People’s Republic of China;
2Department of Rheumatology, The First Affiliated Hospital of Jinan University, Guangzhou, Guandong, People’s Republic of China;
3Department of Rheumatology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guandong, People’s Republic of China
These authors contributed equally to this work
Background: The aggressive phenotype of fibroblast-like synoviocytes (FLSs) is essential in the synovitis and bone destruction in rheumatoid arthritis (RA). Punicalagin is a natural polyphenol extracted in pomegranate juice, which possesses antioxidant, anti-inflammatory and anti-tumor properties suggesting it may be a potent drug for RA therapy. However, there is paucity of information on its effect in RA. ....

United States , San Leandro , Eteläuomen Läi , San Diego , Thiazol Tetrazolium Cytotoxicity , Alpha Innotech , National Institutes Of Health , Jiancheng Bioengineering Institute , American College Of Rheumatology , Laboratory Animal , Cell Signaling Technology , Laboratory Animal Company , Ethics Committee , Technology Co , Us National Institutes Of Health , Nuclear Factor Kappa , American College , Clinical Research Ethics Committee , First Affiliated Hospital , Sun Yat Sen University , Animal Care , Imaging Kit , Pro Inflammation Cytokines , Assay Diluent , Wash Buffer , Healing Assay ,

Inhibition of Long Non-Coding RNA KCNQ1OT1 Attenuates Neuroinflammatio

Knocking Down KCNQ1OT1 Suppressed LPS-Induced Neuroinflammation and Neuronal Apoptosis in HMC3 Cells
For further exploring the function of KCNQ1OT1, we transfected HMC3 cells with pc-KCNQ1OT1 or si-KCNQ1OT1. The expression of KCNQ1OT1 was evaluated by RT-qPCR. Compared with control cells (cells transfected with pc-NC or si-NC), the expression of KCNQ1OT1 in HMC3 cells transfected with pc-KCNQ1OT1 was significantly increased, while the expression of KCNQ1OT1 in cells transfected with si-KCNQ1OT1 was significantly decreased (Figure 2A). Subsequently, we used RT-qPCR and ELISA kits to examine the expressions of TNF-α, IL-1β and IL-6 to evaluate the inflammatory response. The results indicated that LPS treatment markedly promoted the expression levels of inflammatory cytokines TNF-α, IL-1β and IL-6 in HMC3 cells; overexpression of KCNQ1OT1 promoted the expressions of inflammatory cytokines, while KCNQ1OT1 knockdown inhibited their expression (Figure 2B and C). The expression o ....

United States , Chinese Academy Of Medical Science Shanghai , Spss Inc , Biotek Instruments , Promega Corp , Cell Bank , Cytotoxicity Test Kit Jiancheng Bioengineering Institute , Chinese Academy , Medical Science , Modified Eagle , Primer Sequences , Linked Immunosorbent Assay , Molecular Devices , Test Kit , Jiancheng Bioengineering Institute , Nitric Oxide , Reactive Oxygen Species , Beyotime Biotechnology , Induced Neuroinflammation , Neuronal Apoptosis , Inhibitory Effects , Inhibitory Effect , Knocking Down , ஒன்றுபட்டது மாநிலங்களில் , ஸ்ப்ஸ் இன்க் , பயோடெக் கருவிகள் ,