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Losing Winter Ice Is Changing The Great Lakes Food Web Here's How Light Is Shaping Life Underwater

Losing Winter Ice Is Changing The Great Lakes Food Web Here's How Light Is Shaping Life Underwater
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Lake Erie , Canada General , Great Lakes , Brittany Zepernick , Steven Wilhelm , Us Department Of Energy , Genome Institute , Great Lakes Environmental Research Laboratory , Great Lake , Canadian Coast Guard , Canadian Coast Guard Ship Griffon , Joint Genome Institute , Lakes Environmental Research Laboratory ,

Cell envelope and stress-responsive pathways underlie an evolved oleaginous Rhodotorula toruloides strain multi-stress tolerance | Biotechnology for Biofuels and Bioproducts

The red oleaginous yeast Rhodotorula toruloides is a promising cell factory to produce microbial oils and carotenoids from lignocellulosic hydrolysates (LCH). A multi-stress tolerant strain towards four major inhibitory compounds present in LCH and methanol, was derived in our laboratory from strain IST536 (PYCC 5615) through adaptive laboratory evolution (ALE) under methanol and high glycerol selective pressure. Comparative genomic analysis suggested the reduction of the original strain ploidy from triploid to diploid, the occurrence of 21,489 mutations, and 242 genes displaying copy number variants in the evolved strain. Transcriptomic analysis identified 634 genes with altered transcript levels (465 up, 178 down) in the multi-stress tolerant strain. Genes associated with cell surface biogenesis, integrity, and remodelling and involved in stress-responsive pathways exhibit the most substantial alterations at the genome and transcriptome levels. Guided by the suggested stress response ....

Atpase Neo , Genome Institute , Additional File , Supplementary Table , Differentially Expressed Genes , Joint Genome Institute , Eukaryotic Orthologous Groups , Functional Enrichment Analysis ,