Live Breaking News & Updates on Maestro Greenevagreen

G) The mRNA and (
H,
I) protein levels of CCND1 were determined by Real-Time qPCR and Western Blot analysis, respectively. (Note: “Con” indicated “Control”, “KD-L” indicated “Knock-down of LncRNA SNHG16” and “KD-L+KD-miR” suggested “both LncRNA SNHG16 and miR-17-5p ablation”). Each experiment repeated at least 3 times, and
P 0.05.
Knock-Down of LncRNA SNHG16 Inhibited OSCC Progression Through the miR-17-5p/CCND1 Axis
We next investigated whether LncRNA SNHG16 regulated OSCC development by targeting the miR-17-5p/CCND1 axis. To achieve this, the LncRNA SNHG16 silencing vectors, miR-17-5p inhibitor and CCND1 overexpression vectors were delivered into OSCC cells (Figure 6A and B), which were divided into four groups, including Control, KD-SNHG16, KD-SNHG16+miR-17-5p inhibitor, and KD-SNHG16+OE-CCND1. As expected, the data in Figure 6C and D showed that knock-down of LncRNA SNHG16 inhibited cell proliferation in OSCC cells, which were r ....

Eteläuomen Läi , United States , Corning Co , Central South University , Chinese Academy Of Sciences Shanghai , Life Technology , Cell Bank , Animal Center , Xiangya Medical College , Central South University No , Haikou Hospital Of Xiangya Medical College , Affiliated Haikou Hospital , Central South , Western Blot , Ethics Committee , American Type Culture Collection , Chinese Academy , Modified Eagle , Sangon Biotech , Transcription Kit , Applied Biosystems , Maestro Greenevagreen , Primers Used , Beyotime Biotechnology , Antibodies Used , Corning Co Star ,