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[Full text] Silencing of LncRNA SNHG16 Downregulates Cyclin D1 (CCND1) to Abrogate


G) The mRNA and (
H,
I) protein levels of CCND1 were determined by Real-Time qPCR and Western Blot analysis, respectively. (Note: “Con” indicated “Control”, “KD-L” indicated “Knock-down of LncRNA SNHG16” and “KD-L+KD-miR” suggested “both LncRNA SNHG16 and miR-17-5p ablation”). Each experiment repeated at least 3 times, and
P 0.05.
Knock-Down of LncRNA SNHG16 Inhibited OSCC Progression Through the miR-17-5p/CCND1 Axis
We next investigated whether LncRNA SNHG16 regulated OSCC development by targeting the miR-17-5p/CCND1 axis. To achieve this, the LncRNA SNHG16 silencing vectors, miR-17-5p inhibitor and CCND1 overexpression vectors were delivered into OSCC cells (Figure 6A and B), which were divided into four groups, including Control, KD-SNHG16, KD-SNHG16+miR-17-5p inhibitor, and KD-SNHG16+OE-CCND1. As expected, the data in Figure 6C and D showed that knock-down of LncRNA SNHG16 inhibited cell proliferation in OSCC cells, which were r ....

Eteläuomen Läi , United States , Corning Co , Central South University , Chinese Academy Of Sciences Shanghai , Life Technology , Cell Bank , Animal Center , Xiangya Medical College , Central South University No , Haikou Hospital Of Xiangya Medical College , Affiliated Haikou Hospital , Central South , Western Blot , Ethics Committee , American Type Culture Collection , Chinese Academy , Modified Eagle , Sangon Biotech , Transcription Kit , Applied Biosystems , Maestro Greenevagreen , Primers Used , Beyotime Biotechnology , Antibodies Used , Corning Co Star ,

Scientists said claims about China creating the coronavirus were misleading. They went viral anyway.


Scientists said claims about China creating the coronavirus were misleading. They went viral anyway.
Craig Timberg, The Washington Post
Feb. 12, 2021
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Dr. Li-Meng Yan s assertion that China created the coronavirus in a lab in Wuhan was quickly challenged by major scientific journals.Photo for The Washington Post by Jackie Molloy f
Scientists from Johns Hopkins, Columbia and other leading American universities moved with rare speed when a Chinese virologist, Li-Meng Yan, published an explosive paper in September claiming that China had created the deadly coronavirus in a research lab.
The paper, the American scientists concluded, was deeply flawed. And a new online journal from MIT Press - created specifically to vet claims related to covid-19 - reported Yan s claims were at times baseless and are not supported by the data just 10 days after she posted them. ....

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