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Ludwig Johns Hopkins researchers develop new DNA detection method to improve liquid biopsies


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Ludwig Johns Hopkins researchers develop new DNA detection method to improve liquid biopsies
May 6, 2021, NEW YORK – A team of researchers at the Ludwig Center at Johns Hopkins has developed a far more sensitive and accurate next-generation gene sequencing technology for the detection of rare fragments of mutated DNA shed by tumors into body fluids.
The detection of such DNA fragments-commonly referred to as “liquid biopsies”-holds the promise of easing the detection of cancers at their earliest stages, when they are most likely to be curable.
In a study led by Ludwig Johns Hopkins researchers Bert Vogelstein, Kenneth Kinzler, Nickolas Papadopoulos and MD-PhD student Joshua Cohen and published May 3 in Nature Biotechnology, the team reported the development and analytical assessment of SaferSeqS (for “Safer Sequencing System”). Their report shows that SaferSeqS is a major improvement over a now widely adopted method developed at Ludwig Johns Hop ....

Kenneth Kinzler , Ludwig Johns Hopkins , Johns Hopkins , Joshua Cohen , Nickolas Papadopoulos , Bert Vogelstein , Ludwig Center At Johns Hopkins , Ludwig Center , Nature Biotechnology , Safe Sequencing , Ludwig Johns , லுட்விக் ஜான்ஸ் ஹாப்கின்ஸ் , ஜான்ஸ் ஹாப்கின்ஸ் , ஜோஷுவா கோஹன் , நிக்கோலாஸ் பாப்படொபௌஉலோஸ் , பெர்ட் வோகல்ஸ்டீன் , லுட்விக் மையம் இல் ஜான்ஸ் ஹாப்கின்ஸ் , லுட்விக் மையம் , இயற்கை உயிரி தொழில்நுட்பவியல் ,

Hopkins-led research team takes gene mutation detection in blood to the next level


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IMAGE: New SaferSeqS technology detects rare mutations in blood in a highly efficient manner and reduces the error rate.
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Credit: Elizabeth Cooke
Next-generation gene sequencing (NGS) technologies in which millions of DNA molecules are simultaneously but individually analyzed theoretically provides researchers and clinicians the ability to noninvasively identify mutations in the blood stream. Identifying such mutations enables earlier diagnosis of cancer and can inform treatment decisions. Johns Hopkins Kimmel Cancer Center researchers developed a new technology to overcome the inefficiencies and high error rates common among next-generation sequencing techniques that have previously limited their clinical application.
To correct for these sequencing errors, the research team from the Ludwig Center and Lustgarten Laboratory at the Johns Hopkins Kimmel Cancer Center developed SaferSeqS (Safer Sequencing System), a major improvement ....

United States , Janine Ptak , Cristian Tomasetti , Maria Popoli , Joshua Cohen , Jonathanc Dudley , Nickolas Papadopoulos , Bert Vogelstein , Johns Hopkins Christopher Douville , Kenneth Kinzler , Peter Gibbs , Lisa Dobbyn , Natalie Silliman , Johns Hopkins University , Translational Research Grant , National Institutes Of Health , John Templeton Foundation , Lustgarten Foundation For Pancreatic Cancer Research , Dk Ludwig Fund For Cancer Research , Lustgarten Laboratory , Johns Hopkins Kimmel Cancer Center , Sciences In Jan , Eliza Hall Institute Of Medical Research , Ludwig Center , Marcus Foundation , University Of Melbourne ,