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New RNA-sequencing method can help detect numerous modified small RNAs


New RNA-sequencing method can help detect numerous modified small RNAs
A team led by a biomedical scientist at the University of California, Riverside, has developed a new RNA-sequencing method Panoramic RNA Display by Overcoming RNA Modification Aborted Sequencing, or PANDORA-seq that can help discover numerous modified small RNAs that were previously undetectable.
RNA plays a central role in decoding the genetic information in DNA to sustain an organism s life. It is generally known as the intermediate molecule used to synthesize proteins from DNA. Cells are full of RNA molecules in complex and diverse forms, two main types being ribosomal RNA, or rRNA; and transfer RNA, or tRNA; which are involved in the synthesis of proteins. ....

United States , Qi Chen , Sihem Cheloufi , Reuben Franklin , Junchao Shi , Emily Henderson , University Of California , School Of Medicine , Reno School Of Medicine , University Of Nevada , Aborted Sequencing , Nature Cell , Reno School , Assistant Professor , ஒன்றுபட்டது மாநிலங்களில் , குய் சென் , ரூபன் பிராங்க்ளின் , எமிலி ஹென்டர்சன் , பல்கலைக்கழகம் ஆஃப் கலிஃபோர்னியா , பள்ளி ஆஃப் மருந்து , ரெனோ பள்ளி ஆஃப் மருந்து , பல்கலைக்கழகம் ஆஃப் நெவாடா , இயற்கை செல் , ரெனோ பள்ளி , உதவியாளர் ப்ரொஃபெஸர் ,

New RNA-Sequencing Method Opens a Pandora's Box of Opportunity


Panoramic RNA Display by Overcoming RNA Modification Aborted Sequencing (PANDORA) is a new RNA sequencing method developed by scientists from the University of California, Riverside (UCR). Unlike Pandora’s box which refers to a source of endless complications or trouble arising from a single, simple miscalculation this Pandora, may be a source of opportunity as it can help discover numerous modified small RNAs that were previously undetectable.
The researchers have published their method and findings in the journal 
“Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results,” wrote the researchers. “This is partially due to RNA modifications that interfere with adapter ligation and reverse transcription processes, which prevent the detection of sncRNAs bearing these modifications. Here, we present PANDO ....

United States , Qi Chen , Sihem Cheloufi , Reuben Franklin , University Of California , School Of Medicine , Reno School Of Medicine , University Of Nevada , Aborted Sequencing , Unlike Pandora , Nature Cell Biology , Reno School , ஒன்றுபட்டது மாநிலங்களில் , குய் சென் , ரூபன் பிராங்க்ளின் , பல்கலைக்கழகம் ஆஃப் கலிஃபோர்னியா , பள்ளி ஆஃப் மருந்து , ரெனோ பள்ளி ஆஃப் மருந்து , பல்கலைக்கழகம் ஆஃப் நெவாடா , போலல்லாமல் பண்டோரா , இயற்கை செல் உயிரியல் , ரெனோ பள்ளி ,