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First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America | Parasites & Vectors

Trypanosoma evansi infects a large number of wild and domestic animals and causes a spoliative disease known as surra. It is mechanically transmitted, mainly by biting flies of the genera Tabanus and Stomoxys. The detection of T. evansi DNA in the feeding apparatus of Dichelacera alcicornis and Dichelacera januarii from South America is reported, to the best of our knowledge, for the first time. Tabanids were collected weekly from February 2018 to February 2019 from two sites. The feeding apparatus was removed and DNA extraction, polymerase chain reaction and sequencing were performed. A 205-base pair fragment of the variant surface protein RoTat 1.2 gene, confirmed by DNA sequencing, was amplified from the feeding apparatus of D. alcicornis and D. januarii. This is, to the best of our knowledge, the first record of T. evansi DNA in South American tabanids. ....

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Dr. Lee Merritt: SARS-CoV-2 is a genetically engineered BIOWEAPON

Dr. Lee Merritt believes SARS-CoV-2, the virus behind the Wuhan coronavirus (COVID-19) pandemic, is a genetically engineered bioweapon. "SARS-CoV-2 is a genetically engineered bioweapon, and [.] ....

United States , Lee Merritt , Crucell Holland , Davide Martin , National Center , University Of Iowa Research Foundation , Us Department Of Health , Human Services , World Health Organization , University Of Hong Kong , Trademark Office , Children Medical Center Corporation , Genome Sciences Inc , Amy Research , Dana Farber Cancer Institute , Natural News , Bill Gates Funded , Basic Local Alignment Search Tool , Biotechnology Information , Medical Center Corporation , Genome Sciences , Hong Kong , Iowa Research Foundation , Bad Science , Biological Agents And Warfare , Infectious Diseases ,

Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93 | Biotechnology for Biofuels and Bioproducts

The Carbohydrate-Active enZymes (CAZy) auxiliary activity family 3 (AA3) comprises flavin adenine dinucleotide-dependent (FAD) oxidoreductases from the glucose–methanol–choline (GMC) family, which play auxiliary roles in lignocellulose conversion. The AA3 subfamily 1 predominantly consists of cellobiose dehydrogenases (CDHs) that typically comprise a dehydrogenase domain, a cytochrome domain, and a carbohydrate-binding module from family 1 (CBM1). In this work, an AA3 1 gene from T. myriococcoides CBS 398.93 encoding only a GMC dehydrogenase domain was expressed in Aspergillus niger. Like previously characterized CDHs, this enzyme (TmXdhA) predominantly accepts linear saccharides with β-(1 → 4) linkage and targets the hydroxyl on the reducing anomeric carbon. TmXdhA was distinguished, however, by its preferential activity towards xylooligosaccharides over cellooligosaccharides. Amino acid sequence analysis showed that TmXdhA possesses a glutamine at t ....

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Human case of swine-origin influenza A virus detected in Denmark, according to CDC study

Researchers detected a swine influenza A virus (IAV)-infected patient in routine surveillance at the National Influenza Center in Denmark. The detected influenza variant appeared distinct from any variant found previously in Denmark. ....

Aimee Molineux , Neha Mathurnov , Neha Mathur , National Influenza Center , Danish National Influenza Center , Emerging Infectious Disease Journal , Severe Human Case , Zoonotic Infection , Swine Origin Influenza , Genomics Industry Focus , Image Credit , Basic Local Alignment Search Tool ,

Thelazia lacrymalis in horses from Romania: epidemiology, morphology and phylogenetic analysis | Parasites & Vectors

Equine thelaziosis is a neglected vector-borne parasitic disease in modern veterinary medicine, lacking recent reports. It is transmitted by Musca autumnalis, and potentially other Muscidae species, by ingesting the lachrymal secretions of its equine host. The distribution of both Thelazia lacrymalis and its intermediate hosts remains largely unknown throughout Europe, with most studies dating back 20 years. The aim of this study was to assess the presence, prevalence and distribution of T. lacrymalis in horses from Romania. The eyes of 273 horses, slaughtered at two abattoirs from the Northwestern and Western regions of Romania, were examined for the presence of T. lacrymalis between March and November 2021. Upon detection, the nematodes were collected and morphologically identified using the keys from literature. Following identification, one specimen from each animal was selected for molecular analysis while the rest underwent detailed morphometric measurements. Mapping and ....

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