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Sequencing of hsp70 for discernment of species from the Leishmania (Viannia) guyanensis complex from endemic areas in Colombia | Parasites & Vectors

Colombia is ranked very high among countries with the highest numbers of endemic Leishmania species (n = 9) causing human disease. Although much effort has been devoted to generating simple and specific tools for Leishmania species identification, challenges remain in the discrimination of species belonging to the Leishmania (Viannia) guyanensis complex: L. (V.) guyanensis and L. (V.) panamensis. A set of seven reference strains of species belonging to the L. (Leishmania) and L. (Viannia) subgenera, clinical strains from human cases of cutaneous leishmaniasis (CL; n = 26) and samples collected from sylvatic mammals and sand flies (n = 7) from endemic areas in Colombia were analyzed in this study. The heat-shock protein 70 gene (hsp70) was amplified by PCR from DNA extracted from logarithmic-phase promastigotes or tissue samples, and the PCR products were sequenced. Sequence alignment was performed against a set of previously published and curated seq ....

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Moderna patented a 19-nucleotide sequence in 2013 that matches the most infectious sequence of SARS-CoV-2

In the quest to uncover the origins for SARS-CoV-2, scientists did not have to look very far. For years, the National Institutes of Health, in cooperation with EcoHealth Alliance and the [.] ....

Susan Whoriskey , Kenechi Ejebe , Stephane Bancel , Paul Hatala , Anthony Fauci , Atanu Roy , Jasonp Schrum , Jeff Lynn Ellsworth , Saydam Elbashir , Kristym Wood , Matthias John , Tirtha Chakraborty , National Institutes Of Health , Melinda Gates Foundation , University Of North Carolina , Justin Guild , Moderna Therapeutics Inc , Ecohealth Alliance , Modernatx Inc , Wuhan Institute Of Virology , Natural News , National Institutes , North Carolina , Wuhan Institute , Operation Warp Speed , Potential Recombination Link ,

Back when you were distracted by the War in Ukraine, Documents were published confirming Moderna created the COVID Virus

On February 23 the Daily Mail ran an article showing that Moderna has patented the 19 base letter (nucleotide) sequence which codes for the Furin Cleavage site in Covid-19. (Article republished [.] ....

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First genetic report of Ixodes kashmiricus and associated Rickettsia sp. | Parasites & Vectors

Hard ticks (Ixodidae) are hematophagous ectoparasites that transmit various pathogens to a variety of hosts including humans. Transhumant herds have been involved in the spread of ticks and associated Rickettsia spp., and studies on this neglected topic have been unexplored in many regions including Pakistan. This study aimed to investigate ticks infesting transhumant herds of sheep (Ovis aries) and goats (Capra hircus) in district Shangla, Khyber Pakhtunkhwa, Pakistan. Of the 144 examined animals, 112 hosts (68 sheep and 44 goats) of transhumant herds were infested by 419 ticks of different life stages including nymphs (105; 25%), males (58; 14%) and females (256; 61%). For molecular analyses, DNA was extracted from 64 collected ticks and subjected to PCR for the amplification of tick 16S rDNA and ITS2 partial sequences and for the amplification of rickettsial gltA and ompA gene sequences. All tick specimens were identified as Ixodes kashmiricus based on morphological features. The ob ....

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Molecular screening reveals non-uniform malaria transmission in western Kenya and absence of Rickettsia africae and selected arboviruses in hospital patients | Malaria Journal

In sub-Saharan Africa, malaria is the common diagnosis for febrile illness and related clinical features, resulting in the under-diagnosis of other aetiologies, such as arboviruses and Rickettsia. While these may not be significant causes of mortality in malaria-endemic areas, they affect the daily life and performance of affected individuals. It is, therefore, important to have a clear picture of these other aetiologies to institute correct diagnoses at hospitals and improve patient outcomes. Blood samples were collected from patients with fever and other clinical features associated with febrile illness at selected hospitals in the malaria-endemic counties of Busia, Bungoma, and Kakamega, and screened for Crimean-Congo haemorrhagic fever, Sindbis, dengue and chikungunya viruses, Rickettsia africae, and Plasmodium spp. using high-throughput real-time PCR techniques. A logistic regression was performed on the results to explore the effect of demographic and socio-economic independent v ....

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