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Persian buttercup (Ranunculus asiaticus L.) and poppy anemone (Anemone coronaria L.) are ornamental, outcrossing, perennial species belonging to the Ranunculaceae family, characterized by large and highly repetitive genomes. We applied K-seq protocol in both species to generate high-throughput sequencing data and produce a large number of genetic polymorphisms. The technique entails the application of Klenow polymerase-based PCR using short primers designed by analyzing k-mer sets in the genome sequence. To date the genome sequence of both species has not been released, thus we designed primer...

Colombia is ranked very high among countries with the highest numbers of endemic Leishmania species (n = 9) causing human disease. Although much effort has been devoted to generating simple and specific tools for Leishmania species identification, challenges remain in the discrimination of species belonging to the Leishmania (Viannia) guyanensis complex: L. (V.) guyanensis and L. (V.) panamensis. A set of seven reference strains of species belonging to the L. (Leishmania) and L. (Viannia) subgenera, clinical strains from human cases of cutaneous leishmaniasis (CL; n = 2...

Chicken anemia virus (CAV), which has been reported in many countries, causes severe anemia and immunosuppression in chickens. In this study, a CAV strain YN04 belonging to genotype A was firstly identified from infected chickens in Yunnan province, China. Moreover, the animal infection experiments further confirmed that the strain YN04 is a highly pathogenic strain, which can cause 86.67% mortality of chickens in infection group. The mean death time of infected chickens was 13.1 days post infection (dpi). CAV infection induced severe anemia with significant decrease of packed cell volume, and...

↓ cleavage residues indicate that the NDV isolates correspond to mesogenic/velogenic pathotypes. Figure 2 Deduced amino acid sequence alignment of F gene sequences of NDV strains isolated from Ethiopia. Names of strains are shown on the left-hand side of the figure. Identification with the reference La Sota (genotype II) vaccine strains is indicated by dots. They indicate the cleavage site of the deduced aa sequence of the F gene. X indicates an ambiguous aa sequence. Abbreviations: Eth, Ethiopia; Chk, chicken. Genetic Distance The Ethiopian NDV isolates have a within-group genetic distance...