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Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L. plantarum was optimized for PMA treatment concentration, PMA detection sensitivity and multiple con...

Climate change, pest infestation, and soil degradation are significantly reducing wheat (Triticum aestivum L.) yield. Wheat is cultivated in rice-wheat and cotton-wheat cropping systems and escalating global population is exerting substantial pressure on the efficiency of these systems. Conservation tillage and crop rotation could help in lowering soil degradation and pest infestation, and improving wheat yield. This three-year study evaluated soil properties, weed infestation and wheat yield under various tillage and cropping systems. Six different cropping systems, i.e., cotton-wheat, sorghu...

Common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learning the rhizobial communities associated with common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (Cuican and Jiadouwang) grown in two sites of blackland, and were characterized by comparative sequence analyses of 16S rRNA, recA, atpD, nodC, nifH genes and whole genome. As results, Rhizobium indigoferae, R. anhuiense and R. croatiense as minor groups and three dominant novel Rhizobium species were ide...

Aflatoxins (AFT) identified as a Group 1 human carcinogen naturally contaminate various types of food and could increase the risk of hepatocellular carcinoma (HCC) through dietary intake. Chongqing municipality is located in Southwest China with subtropical monsoon climate which is conducive to AFT contamination in crops. However, the burden of HCC caused by the dietary exposure of the population in Chongqing to AFT has not been quantified. We estimated the burden of HCC in terms of Disability Adjusted Life Year (DALY) using FDA-iRISK software. Dietary exposure to AFT in three food categories ...

Agri-food value chains that generate an increased willingness to pay among consumers by offering enhanced levels of sustainability are widely discussed as an important strategy for creating new business opportunities and fostering food system transformation. Previous research has highlighted the importance of governance arrangements to secure the trust necessary for the establishment of sustainability-based agri-food value chains. However, how different coordination designs by private and public actors along the value chain affect trust formation is not well understood. To address this questio...