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Frontiers | Diversity analyses in two ornamental and large-genome Ranunculaceae species based on a low-cost Klenow NGS-based protocol - Vimarsana News

Frontiers | Diversity analyses in two ornamental and large-genome Ranunculaceae species based on a low-cost Klenow NGS-based protocol

Persian buttercup (Ranunculus asiaticus L.) and poppy anemone (Anemone coronaria L.) are ornamental, outcrossing, perennial species belonging to the Ranunculaceae family, characterized by large and highly repetitive genomes. We applied K-seq protocol in both species to generate high-throughput sequencing data and produce a large number of genetic polymorphisms. The technique entails the application of Klenow polymerase-based PCR using short primers designed by analyzing k-mer sets in the genome sequence. To date the genome sequence of both species has not been released, thus we designed primer...

Frontiers | Isolation of Brucella inopinata from a White's tree frog (Litoria caerulea): pose exotic frogs a potential risk to human health? - Vimarsana News

Frontiers | Isolation of Brucella inopinata from a White's tree frog (Litoria caerulea): pose exotic frogs a potential risk to human health?

Cold-blooded hosts, particularly exotic frogs, have become a newly recognized reservoir for atypical Brucella species and strains worldwide, but their pathogenicity to humans remains largely unknown. Here we report the isolation of a B. inopinata strain (FO700662) from a diseased White's Tree Frog (Litoria caerulea) in Switzerland. Originally identified as Ochrobactrum based on its rapid growth and biochemical profile, strain FO700622 was positive for the Brucella-specific markers bcsp31 and IS711. It showed the specific banding pattern of B. inopinata in conventional Bruce-ladder multiple...

Frontiers | Harnessing solar and wind for sustainable cross-border electricity trade in the Greater Mekong Subregion - Vimarsana News

Frontiers | Harnessing solar and wind for sustainable cross-border electricity trade in the Greater Mekong Subregion

• Sustainable, low-emission electricity generation options are needed in the Greater Mekong Subregion, including for cross-border electricity trade • Large-scale investment in solar and wind power, together with off-river pumped hydro energy storage, is identified as a promising way forward • The GMS has many potential off-river pumped hydro sites that could provide ample energy storage • Actionable recommendations include greater use of bilateral power purchase agreements for cross-border solar and wind power supply, and potential development of a high-voltage direct current grid • ...

Frontiers | Antibacterial and anti-inflammatory effects of PGLa-loaded TiO2 nanotube arrays - Vimarsana News

Frontiers | Antibacterial and anti-inflammatory effects of PGLa-loaded TiO2 nanotube arrays

Objectives:This study investigated the antimicrobial effect and anti-inflammatory activities of PGLa-loaded TiO2 nanotube arrays (TiO2 NTs) in osteoblast-like MG-63 cells. Methods:The surface morphology and roughness of three titanium (Ti) substrates (Ti, TiO2 NTs, PGLa-loaded TiO2 NTs) were evaluated by scanning electron microscopy (SEM) and atomic force microscope (AFM). The wettability of three titanium substrates was evaluated by contact angle. Biocompatibility of PGLa-loaded TiO2 NTs were evaluated in MG-63 cells (cell adhesion, proliferation, cytoskeletal evaluation and alkaline phos...

Frontiers | A study of the correlation between stroke and gut microbiota over the last 20years: a bibliometric analysis - Vimarsana News

Frontiers | A study of the correlation between stroke and gut microbiota over the last 20years: a bibliometric analysis

Purpose: This study intends to uncover a more thorough knowledge structure, research hotspots, and future trends in the field by presenting an overview of the relationship between stroke and gut microbiota in the past two decades. Method: Studies on stroke and gut microbiota correlations published between 1st January 2002 and 31st December 2021 were retrieved from the Web of Science Core Collection and then visualised and scientometrically analysed using CiteSpace V. Results: A total of 660 papers were included in the study, among which the USA, the UK, and Germany were the leading research ce...