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Frontiers | A rapid and reliable method for the determination of Lactiplantibacillus plantarum during wine fermentation based on PMA-CELL-qPCR

Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L. plantarum was optimized for PMA treatment concentration, PMA detection sensitivity and multiple conditions of sample pretreatment in wine environment, and the optimized method can accurately quantify 10 4 -10 8 CFU/mL of the target strain (L. plantarum) in multiple matrices; (2) when the concentration of dead bacteria in the system is 10 4 times higher than the concentration of live bacteria, there is an error of 0.5-1 lg CFU/mL in the detection results. The optimized sample pretreatment method ....

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Frontiers | Predicting Spike Features of Hodgkin-Huxley-Type Neurons With Simple Artificial Neural Network

Hodgkin-Huxley (HH) -type model is the most famous computational model for simulating neural activity. It shows the highest accuracy in capturing neuronal spikes, and its model parameters have definite physiological meanings. However, HH-type models are computationally expensive. To address this problem, a previous study proposed a spike prediction module (SPM) to predict whether a spike will take place 1 ms later based on three voltage values with intervals of 1ms. Although SPM does well, it fails to evaluate the informative features of the spike. In this study, the feature prediction module (FPM) based on simple ANN was proposed to predict spike features including maximum voltage, minimum voltage, and dropping interval. Nine different HH-type models were adopted whose firing patterns cover most of the firing behaviors observed in the brain. Voltage and spike feature samples under constant external input current were collected for training and testing. Experiment results illustrated t ....

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